摘要
目的：建立测定安神宝颗粒中斯皮诺素含量的高效液相色谱方法。方法采用Phenomenex LUNA-C18柱（4.6 mm×250 mm，5μm），以乙腈-0.005 mol/L磷酸二氢钾溶液（19∶81）为流动相，流速1.0 ml/min，柱温30℃，检测波长334 nm。结果斯皮诺素在1.83～36.60μg/ml浓度范围内有良好线性关系（r＝0.9998），平均加样回收率为98.4%，RSD为1.22%。结论本方法简便，快速，准确，重复性好，可用于安神宝颗粒中斯皮诺素的含量测定。
Objective To establish a HPLC method for the determination of Spinosin in anshenbao granule. Methods The HPLC was adopted with Phenomenex LUNA-C18（4.6 mm×250 mm, 5μm）column, and the mobile phase consisted of Acetonitrile（19）-0.005 mol/L Kaliumdihydrogenphosphorate（81）. The flow rate was 1.0 ml/min, the column temperature was 30℃ and the UV detector was set at 250 nm. Results The linear response range was 1.83~36.60μg/ml（r=0.9998）. The average recovery of spinosin was 98.4%, and RSD1.22%. Conclusion The method was simple, rapid, accurate and repeatable. It can be applied in determination of spinosin in Anshenbao granule.
出处
《国际中医中药杂志》
2014年第5期442-444,共3页
International Journal of Traditional Chinese Medicine
