摘要
目的 筛选对慢性、轻度不可预见性应激（CUMS）敏感的小鼠品系,并初步研究其敏感机制.方法 选用KM、ICR、BABL/c、C57BL/6小鼠建立CUMS抑郁模型,通过体质量、糖水偏好、旷场实验得分考察不同品系小鼠对CUMS的敏感性.通过对小鼠海马组织糖皮质激素受体（GR）、5-羟色胺（5-HT）、犬尿氨酸（KYN）含量的测定,对其易感机制进行初步研究.结果 造模后C57BL/6小鼠体质量、糖水偏好、旷场实验水平运动和垂直运动得分等指标均对CUMS表现出显著敏感性,BABL/c小鼠对糖水偏好、旷场实验水平运动和垂直运动得分较为敏感.对照组C57BL/6小鼠海马组织GR、KYN含量与其他三种小鼠存在显著差异.结论 在筛选的4个品系小鼠中,C57BL/6小鼠对CUMS最为敏感,适于建立CUMS抑郁症动物模型,其敏感机制可能与海马组织GR、KYN含量有关.
Objective To screen mouse strain that susceptive to chronic unpredicted mild stress （CUMS） and preliminarily research its suscepfive mechanism. Methods KM, ICR, BABL/c and C57BL/6 mice were used to establish CUMS depression model. Body weight, sucrose preference test（SPT）, open field test （OFT） were used to evaluate the sensitivity of mice to CUMS. Hippocampal levels ofglucocor- ticoid reecptor （GR）, 5-hydroxytryptamine（5-HT）, kynurenine（I（YN） were measured and used for susceptive mechanism research. Results Body weight, SPT, horizontal movement score and vertical movement score of C57BL/6 mice were found to susceptive to CUMS. SPT, horizontal movement score and vertical movement score of BABL/c showed similar tendency. Hippocampal levels of GR and KYN of normal C57BL/6 mice were significantly lower or higher than that of normal KM, ICR and BABL/c mice. Conclusions The C57BL/6 mouse is most susceptive to CUMS among 4 strains tested, and hippocampal levels of GR and KYN may be the main mechanism responsible for its susceptibility.
作者
徐龙进
王可洲
XU Long-jin WANG Kou-zhou(Shandong Center for Disease Control and Prevention, Ji＇nan, 250014, China Laboratory Animal Center of Shandong Province, Ji＇nan, 250011, China)
出处
《实验动物与比较医学》
CAS
2016年第4期257-262,共6页
Laboratory Animal and Comparative Medicine
基金
国家自然科学基金（31171219）
关键词
小鼠
慢性应激
易感品系
机制
Mouse
Chronic stress
Susceptive strain
Mechanism
