摘要
背景:有研究发现,将纳米羟基磷灰石与光固化树脂基质制备成复合材料,其压缩强度和挠曲强度能够达到牙科修复材料的要求。目的:检测纳米羟基磷灰石复合树脂材料的细胞毒性,分析其修复牙体缺损的效果。方法:(1)体外实验:采用10%、50%、100%浓度的纳米羟基磷灰石复合树脂材料浸提液分别培养第5代牙周膜成纤维样细胞与L-929细胞,以新鲜细胞维持液的为阴性对照,培养7 d内检测细胞增殖A值。(2)体内修复试验:选择57例牙体缺损患者,其中男37例,女20例,年龄15-31岁,均采用纳米羟基磷灰石复合树脂材料进行修复,修复后随访3年,检查修复体完整性、边缘适合性、颜色匹配情况,以及牙周探诊深度、龈沟出血指数、牙齿松动度、菌斑指数、龈沟液量、天冬氨酸转氨酶水平等。结果与结论:(1)体外实验:不同浓度纳米羟基磷灰石复合树脂材料浸提液对牙周膜成纤维样细胞与L-929细胞的增殖均无影响;(2)体内修复试验:修复后随访3年,患者牙周探诊深度、龈沟出血指数、牙齿松动度、菌斑指数、龈沟液量、天冬氨酸转氨酶水平较修复前显著下降(P<0.05),碱性磷酸酶水平未出现明显改变(P>0.05);出现修复体不完整3例,颜色不匹配4例,边缘不适合3例;(3)结果表明:纳米羟基磷灰石复合树脂材料无细胞毒性,修复牙体缺损可维持良好的牙周健康状态,具有良好的生物相容性。
BACKGROUND： It has been found that the compressive strength and flexural strength of nanohydroxyapatite combined with light-cured resin matrix can neet the requirements of dental restorative materials. OBJECTIVE： To detect the cytotoxicity of nano-hydroxyapatite/composite resin, and to analyze its effects on the dental restoration. METHODS： In vitro test： passage 5 periodontal ligament fibroblast-like cells and L-929 cells suspensions were cultured in the 10%, 50%, 100% of nano-hydroxyapatite/composite resin extracts, respectively, and fresh maintenance medium functioned as negative control group. Absorbance values were detected within 7-day culture. In vivo repair test： 57 cases with tooth defect were enrolled containing 37 males and 20 females aged from 15 to 31 years old, and all were subjected to nano-hydroxyapatite/composite resin treatment. A 3-year follow-up was undergone to detect the completeness, marginal adaptation and color matching of the restoration, as well as the periodontal probing depth, sulcus bleeding index, tooth mobility, dental plaque index, gingival crevicular fluid and aspartate aminotransferase level. RESULTS AND CONCLUSION： In vitro test results showed that the different concentrations of nanohydroxyapatite/composite resin extracts had no effects on both periodontal ligament fibroblast-like cells and L-929 cells. In vivo repair test results found that after 3 years of follow-up, the periodontal probing depth, sulcus bleeding index, tooth mobility, dental plaque index, gingival crevicular fluid and aspartate aminotransferase level of patients were significantly decreased than those before repair（P 〈0.05）, but the alkaline phosphatase level revealed no significant change（P 〉0.05）. Unfortunately, three cases with incomplete restoration, four cases with color mismatching, and three cases with the inadaptable edge occurred. To conclude, the nano-hydroxyapatite/composite resin holds good biocompatibility and no cytotoxicity, which can maintain a good periodontal health
出处
《中国组织工程研究》
CAS
北大核心
2016年第38期5697-5702,共6页
Chinese Journal of Tissue Engineering Research
