摘要
目的 探讨香芹酚对大鼠脊髓损伤（SCI）后神经功能的影响及其机制。方法 将60只雄性SD大鼠（200-250 g）随机分为5组：假手术组（n=12）、SCI组（n=12）、香芹酚组（n=36），香芹酚组根据香芹酚剂量分为低、中、高剂量3个亚组，每亚组12只。低、中、高剂量香芹酚组SCI后30 min腹腔注射香芹酚，剂量分别为10、20、40 mg/kg，每日一次；假手术组和SCI组每日腹腔注射等量生理盐水。采用Allen法建立大鼠SCI模型；假手术组只行椎板切除手术。SCI后24、48、72 h，采用BBB评分评估大鼠神经功能；SCI后72 h，采用ELISA法检测损伤脊髓组织丙二醛（MDA）、超氧化物歧化酶（SOD）、谷胱甘肽（GSH）、过氧化氢酶（CAT）、caspase-3活性；Western-blot法检测损伤脊髓组织Bax，Bcl-2蛋白表达水平。结果 SCI后，大鼠BBB评分均明显降低（P〈0.05），损伤脊髓组织水肿指数以及MDA、caspase-3和Bax水平均明显增高（P〈0.05），而SOD、GSH、CAT、Bcl-2水平均明显降低（P〈0.05）；香芹酚能明显改善大鼠BBB评分（P〈0.05），明显降低水肿指数以及MDA、caspase-3和Bax水平（P〈0.05），而显著增加CAT、SOD、GSH、Bcl-2水平（P〈0.05）。结论 香芹酚可通过减轻脊髓水肿、抑制氧化应激反应以及抗凋亡作用而对SCI大鼠发挥神经保护作用。
Objective To investigate the effects of carvacrol on spinal cord edema, oxidative stress reaction and cell apoptosis after spinal cord injury in rats. Methods Spine cord injury （SCI） at the tenth thoracic vertebrae level was induced by the modified weight-drop method. Sixty SD rats were randomly divided into 5 groups of 12 animals each, i.e. sham operation group （laminectomy was only performed without SCI）, SCI group and experimental groups 1, 2, and 3. Each rat received intraperitoneal injection of 10 mg/kg, 20 mg/kg and 40 mg/kg carvacrol once a day respectively for 3 days in the experimental groups 1, 2 and 3. The motor function of the hind limb was determined by the Basso, Beattie and Bresnahan （BBB） scale 24, 48 and 72 hours after SCI in all the groups, in which water content in injured spinal cord tissues was determined. The malondialdehyde （MDA）, catalase （CAT）, superoxide dismutase （SOD）, and glutathione peroxidase （GSH） were determined in the injured spinal cord tissues, in which the expressions of Bax and Bcl-2 proteins were detected by Western blot. Results BBB Scale scores were significantly higher in all the experimental groups than those in SCI group （P〈0.05）. The water content in the injured spinal cord tissues was significantly less in all the experimental groups than that in SCI group 72 hours after SCI （P〈0.05）. The activities of MDA, CAT, SOD and GSH were signicantly lower in all the experimental groups than those in SCI group 72 hours after SCI （P〈0.05）. The level of Bax expression was significantly lower and the level of Bcl-2 expression was significantly higher in all the experimental groups than those in SCI group （P〈0.05）. Conclusions It is suggested that carvacrol protects the injured spinal cord via suppressing spinal cord edema and oxidative stress reaction and anti-apoptotic pathway.
出处
《中国临床神经外科杂志》
2016年第9期549-552,共4页
Chinese Journal of Clinical Neurosurgery
关键词
脊髓损伤
氧化应激
细胞凋亡
香芹酚
大鼠
Carvacrol
Spinal cord injury
Oxidative stress reaction
Neuroproteetion
