摘要
为了有效的提高灵芝(Ganoderma lucidum)菌丝体液态深层发酵合成灵芝三萜的能力,研究了氧载体正十二烷对灵芝三萜发酵的影响。以灵芝三萜浓度为指标,采用单因素实验对正十二烷的添加量和添加时间进行初步确定,再利用中心组合设计对灵芝菌丝体液态深层发酵中氧载体正十二烷的添加工艺进行优化。结果表明,在发酵的第25.63小时添加29.85mL/L的正十二烷,灵芝三萜浓度理论值为0.84g/L,在500mL摇瓶和5L发酵罐中验证,得到灵芝三萜的浓度分别为0.85g/L和0.88g/L,相比对照组分别提高了578%和398%。
Single factor experiments combined with Central Composite Design methodology revealed that addition of the oxygen vector n-dodecane to submerged cultures of G.lucidum G0119 increased triterpene production.Addition of 29.85 mg n-dodecane per mL of culture after 25.63 h was predicted to give a triterpene yield of 0.84g/L.Under these conditions,actual triterpene yields of 0.85g/L in 500 mL shake flasks and 0.88g/L in a 5-L fermentor were recorded,which represented 578% and 398% increases,respectively compared to the corresponding controls without added n-dodecane.
出处
《食用菌学报》
CSCD
北大核心
2016年第2期52-58,共7页
Acta Edulis Fungi
基金
上海市市级农口系统青年人才成长计划资助项目(沪农青字2015第1-7号)
上海市农业科学院青年科技人员“助跑”计划(ZP06)资助
关键词
灵芝
深层发酵
氧载体
正十二烷
Ganoderma lucidum
submerged culture
oxygen vector
n-dodecane
