摘要
以灵芝菌丝体为原料,在单因素试验基础上,通过正交试验和Box-Behnken响应面设计法优化超声辅助提取灵芝菌丝体总三萜工艺。结果表明,超声辅助提取灵芝菌丝体总三萜正交试验最佳工艺为:超声功率90 W、超声时间50 min、pH 8、料液比1∶40(g/mL),在此条件下灵芝菌丝体三萜平均提取率为0.76%;超声辅助提取灵芝菌丝体总三萜响应面试验最佳工艺为:超声功率90 W、超声时间42 min、pH 8、料液比1∶30(g/mL),在此条件下,灵芝菌丝体总三萜平均提取率为1.09%,与最大预测值1.13%相差0.04%。这两种方法得到的提取工艺参数可靠,可为灵芝三萜工业化生产提供技术参考。
Based on the single factor experiment,the Ganoderma lucidum mycelium was used to optimize the ultrasonic assisted extraction of the total triterpenoids of Ganoderma lucidum mycelium by orthogonal test and Box-Behnken response surface design. The results showed that the optimal process for ultrasonic extraction of total triterpenoids of Ganoderma lucidum mycelium was as follows:ultrasonic power 90 W,ultrasonic time50 min,pH 8,ratio of material to liquid 1∶40(g/mL),under which Ganoderma lucidum hyphae The average extraction rate of body triterpenoids was 0.76 %;the optimal process for ultrasonic-assisted extraction of Ganoderma lucidum mycelium total triterpenoid response surface test was:ultrasonic power 90 W,ultrasonic time 42 min,pH 8,material-to-liquid ratio 1∶30(g/mL),Under these conditions,the average extraction rate of total triterpenoids of Ganoderma lucidum mycelium was 1.09 %,which was 0.04 % different from the maximum predicted value of 1.13 %. The extraction process parameters obtained by these two methods are reliable,and can provide technical reference for the industrial production of Ganoderma lucidum.
作者
刘奇
彭浩
乔伟
邓百万
赵航柯
曹丹
LIU Qi;PENG Hao;QIAO Wei;DENG Bai-wan;ZHAO Hang-ke;CAO Dan(College of Biological Science and Engineering,Shaanxi Sci-Tech University,Hanzhong 723000,Shaanxi,China;Shaanxi Engineering Research Center of Edible and Medicated Fungi,Hanzhong 723000,Shaanxi,China)
出处
《食品研究与开发》
CAS
北大核心
2020年第5期85-92,共8页
Food Research and Development
基金
陕西省重点研发计划资助项目(2017ZDXM-NY-001)
关键词
灵芝菌丝体
总三萜
超声提取
正交试验
响应面试验
Ganoderma lucidum mycelium
total triterpenoids
ultrasonic extraction
orthogonal test
response surface test
