摘要
萘作为最简单的多环芳烃化合物,对水生生物有致死致畸作用,并能破坏生物的抗氧化防御系统.选用模式生物雌性斑马鱼成体和胚胎为受试对象进行了萘暴露处理.斑马鱼胚胎发育从受精后3h开始进行萘溶液染毒处理,处理浓度分别为0(对照组),1.5,2.5和7.5μg/L(3个处理组),至受精后96h结束.染毒期间观察胚胎发育的毒理学终点.成年雌性斑马鱼暴露在0,84,840μg/L的萘溶液中96h.研究结果表明,萘对斑马鱼胚胎具有发育毒性,受精后24h凝结率及中枢神经发育异常,有明显的浓度依赖性.甲基化敏感扩增多态性(MSAP)方法检测肝脏、脑和肌肉组织受萘胁迫后DNA甲基化水平变化的结果表明,肝脏和脑中DNA甲基化变化水平较肌肉组织高.在肝脏中,DNA甲基化变化水平较对照组分别高出12.88%(84μg/L处理组)和8.16%(840μg/L处理组).
The naphthalene as the simplest PAHs（polycyclic aromatic hydrocarbons）,is considered to be lethal and teratogenic effects on aquatic organism,also refers to the disruption of antioxidant defense system function.In the present study,adult female zebrafish（Danio rerio）and embryo（Tubingen line）were used to naphthalene exposure tests.Zebrafish embryos were exposed to different concentrations of naphthalene（0,1.5,2.5,7.5μg/L）from 3hpost-fertilization（hpf）to 96 h pf.During the exposure period,embryo developmental endpoints were examined by microscope.The adult zebrafish were exposed to 0,84μg/L and 840μg/L of naphthalene for 96 h.Exposure to naphthalene caused a developmental toxicity,including reduced survival,increased coagulation rates and abnormal 72 hpf response ability in a dose-dependent manner.After the naphthalene exposure,used the MSAP（methylation sensitive amplification polymorphism）analysis to measure the DNA methylation level changes in adult female zebrafish liver,brain and muscle.Higher DNA-methylation level changes showed in the liver and brain than that in muscle.Especially in the liver,DNA methylation level changes in treatment groups are 12.88%（84μg/L）and 8.16%（840μg/L）higher than that in control groups respectively.
出处
《东北师大学报（自然科学版）》
CAS
CSCD
北大核心
2016年第3期167-173,共7页
Journal of Northeast Normal University(Natural Science Edition)
基金
国家水体污染控制与治理科技重大专项项目(2008ZX07207-009-03)
关键词
多环芳烃
萘
斑马鱼
表观遗传
DNA甲基化
MSAP
polycyclic aromatic hydrocarbon
naphthalene
MSAP
zebrafish
epigenetic
DNA methylation
