摘要
建立了生活用纸中乙二醛残留量的分光光度测定方法。乙二醛以甲基红-亚甲基蓝为指示剂,用氢氧化钠溶液标定并绘制标准曲线。试样经水萃取,乙二醛与2-亚肼基-2,3-二氢-3-甲基苯并噻唑盐（HMBT）在乙酸溶液中80℃下反应生成一种黄色化合物,然后用分光光度法在405 nm波长下进行测定,甲醛无干扰。该方法线性相关性系数0.9999,摩尔吸收系数4.78×104L/（mol·cm）,检出限0.20 mg/kg,6次平行测定的标准偏差为3.16%～5.59%,回收率84.75%～91.03%。该方法操作简便,实用性强,选择性、检出限、定量限、线性、灵敏度、精密度和回收率均满足相关技术法规对生活用纸中乙二醛残留量限量的规定。
The method was established to determine glyoxal residue in tissue products by spectrophotometry. Theglyoxal was calibrated by sodium hydroxide solution with methyl red and methylthionine chloride mixed solution as the indicator,and the standard curve was drawn. The sample was extracted with water,glyoxal reacted with 2-hydrazono-2,3-dihydro-3-methyl benzothiazol hydrochlorid（HMBT） in acetic acid solution at 80℃,generating a yellow compound,and then determined at 405 nm wavelength by spectrophotometry,non interference is found by formaldehyde. The linear correlation coefficient is 0. 9999,molar absorption coefficient is 4. 78 × 104 L/（mol·cm）,detection limit is 0. 20 mg/kg,and standard deviation of 6 parallel determination is between 3. 16%～ 5. 59%,the recovery rate is between 84. 75% ～ 91. 03%. This method has the advantage of convenient operation,feasible,and with satisfying selectivity,detection limit,quantification limit,linearity,sensitivity,precision and recovery.
出处
《造纸科学与技术》
2016年第4期35-39,共5页
Paper Science & Technology
基金
国家质检总局检验检疫行业标准制修订计划(2012B104)
