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脂多糖对奶牛乳腺上皮细胞IL-6、IL-8和TNF-α mRNA表达的影响 被引量:4

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摘要 本试验以体外培养的奶牛乳腺上皮细胞为模型,研究不同浓度脂多糖刺激奶牛乳腺上皮细胞时IL-6、IL-8及TNF-α的mRNA表达情况。结果显示,LPS浓度为10μg/mL时刺激效果最好;10μg/mL LPS刺激后24h,IL-6和IL-8的表达量最高(P<0.05),TNF-α的mRNA表达在刺激后的4h达最高(P<0.05)。以上结果说明,本实验室体外培养的奶牛乳腺上皮细胞具有表达与免疫有关的细胞因子功能,可以作为一种模型来研究奶牛乳腺炎的发病机制。
出处 《上海畜牧兽医通讯》 2016年第5期11-15,共5页 Shanghai Journal of Animal Husbandry and Veterinary Medicine
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