摘要
本试验对板蓝根、黄芪的提取物以及青蒿素的萃取液抗猪繁殖与呼吸综合征病毒(PRRSV)的作用机制进行研究。采用光学显微镜观察各细胞株形态上的变化,同时根据试验收集到的不同中草药提取物在不同浓度下(1、2、4、6μg/m L)的细胞保护率,采用SPSS18.0软件对数据进行统计分析。分析结果表明:不同浓度的中草药提取物抑制PRRSV所导致的细胞变性效果有显著差异,浓度越高,抑制效果越好。同时,不同的中草药提取物对PRRSV的抑制效果也存在显著差异,板蓝根提取物具有一定的减少病毒造成细胞病变的作用,而青蒿素提取物保护细胞的能力效果最佳,有效降低PRRS病毒复制与抑制病毒导致细胞变性效果,阻断病毒的复制以降低感染的机会。
In this test, the action of radix, astragalus and artemisinin extract against porcine reproductive and respira- tory syndrome virus（PRRSV） and their mechanism were studied.An optical microscope was used to observe morphological changes in each cell line, and according to test cytoprotective rate collected from different concentrations （1,2,4,6 μg/mL） of herbal extracts,SPSS18.0 statistical software was used for data polarity analysis.The results showed that ：the different concentrations of herbal extracts inhibiting cell degeneration by PRRS virus caused a significant difference,the higher the concentration,the inhibitory effect was better.At the same time,the effect of different herbal extracts on PRRS virus inhibitory was significantly different,radix extract had a certain reduction in viral causing cytopathic phenomenon,and artemisinin extract had the best cells protection,reduced the PRRS virus replication and inhibition effect of the virus that caused the degeneration of cells, block viral replication in order to reduce the chance of infection.
出处
《中国饲料》
北大核心
2016年第17期16-19,共4页
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