摘要
目的 探讨造模前进行不同前期干预后心肌梗死（MI）与抑郁大鼠血清、血小板、脑组织中的5-羟色胺（5-HT）、5-羟色胺2A受体（5HT2AR）及5-羟色胺转运体（SERT）的变化.方法 通过随机数字表法将80只SD大鼠随机分2组,分别予生理盐水或人参果实总皂苷灌胃4周后,每组再随机分为4个亚组,分别建立MI、抑郁、MI合并抑郁与假手术亚组4种动物模型,造模成功3d后处死,观察各组大鼠血清、血小板裂解液及脑组织匀浆中5-HT、5-HT2AR及SERT水平.结果 生理盐水前期干预组中,假手术亚组的血清5-HT水平比MI、抑郁以及MI合并抑郁亚组高[（237.1 ±32.0）pg/ml比（58.0±11.6） pg/ml、（72.8±2.3）pg/ml、（62.5 ± 10.2）pg/ml,P值均＜0.001].人参果实总皂苷前期干预组中,各亚组血清5-HT水平较生理盐水各亚组升高,其中MI、抑郁和MI合并抑郁亚组上升明显（P值均＜0.001）;血小板裂解液5-HT水平在抑郁、MI合并抑郁亚组明显高于生理盐水前期干预组（P值均＜0.05）;血小板裂解液及脑组织匀浆5-HT2AR水平在MI、抑郁、MI合并抑郁亚组均低于生理盐水组各亚组（P值均＜0.05）;血小板裂解液及脑组织匀浆SERT水平与生理盐水组比较有上升趋势,其中抑郁亚组的血小板裂解液SERT上升有统计学意义（P =0.019）,而MI亚组血小板裂解液SERT下降明显（P=0.001）;而脑组织匀浆5-HT及SERT水平与生理盐水组差异无统计学意义（P值均＞0.05）.结论 在不同的刺激因素、心脏生理状态和心理应激状态下5-HT系统的反应性不同.人参果实总皂苷对5-HT系统功能有一定的调控作用,对抑郁、MI合并抑郁作用明确,但对急性MI无明显作用.
Objective To evaluate the effect of ginsenoside pre-treatment on serotonin （5-hydroxytryptamine,5-HT）,5-HT2A receptor （5-HT2A R）,and 5-HT transporter （serotonin transporter,SERT） in serum,platelet lysate and brain tissue homogenates in SD rats with myocardial infarction （MI） or depression.Methods Eighty SD rats were randomized treated with ginsenoside or normal saline （NS）.After 4 weeks,the rats were,then,randomized to four subgroups：the MI,the depression,the MI in combination with depression and sham subgroups.5-HT,5-HT2A R and SERT levels were detected in serum,platelet or brain.Results In the NS pre-treatment groups,serum 5-HT levels in sham rats （237.1 ± 32.0）pg/ml were higher than in MI （58.0 ± 11.6） pg/ml,depression （72.8 ± 2.3） pg/ml,and MI with depression rats （62.5 ± 10.2） pg/ml （all P =0.000）.Pre-treatment of ginsenoside increased serum and platelet 5-HT in MI,depression and MI with depression rats compared with each corresponding NS treated subgroups （all P 〈 0.005）.However,treatment of ginsenoside lowered the 5-HT2A R expression in MI,depression,and MI with depression subgroups both in platelet and brain tissue homogenates compared with each corresponding NS treated subgroups （all P 〈 0.05）.As to SERT in platelet,treatment of ginsenoside increased its levels in depression rats （P =0.019）,but lowered the levels in MI rats （P =0.001） compared with NS treated rats.There were no differences in brain 5-HT and SERT between ginsenoside and NS treated groups.Conclusions The 5-HT system responses differently to ginsenoside treatment under different cardiac and mental stress conditions.Ginsenoside plays an important role in regulation of 5-HT system.
出处
《中华内科杂志》
CAS
CSCD
北大核心
2016年第9期700-704,共5页
Chinese Journal of Internal Medicine
关键词
心肌梗死
抑郁
5-羟色胺系统
Myocardial infarction
Depression
5-Hydroxytryptamine system
