摘要
目的:探讨雌激素对阿尔茨海默病(AD)大鼠学习记忆能力的影响。方法:选取雌性大鼠36只,随机分为假手术组、卵巢切除(OVX)组、OVX+苯甲酸雌二醇(EB)组,每组12只。首先采用海马注射Aβ1-42建立AD模型,然后通过Morris水迷宫观察大鼠的学习记忆能力,同时通过ELISA检测脑组织超氧化物歧化酶(SOD)、丙二醛(MDA)、乙酰胆碱酯酶(Ch AT)的活性。结果:与OVX组比较,OVX+EB组逃避潜伏期明显缩短,差异有统计学意义(P<0.05),原平台象限活动时间明显增加,差异有统计学意义(P<0.05),穿越原平台次数明显增多,差异有统计学意义(P<0.05)。且提高大鼠脑组织SOD、Ch AT活性,降低MDA活性,差异有统计学意义(P<0.05)。结论:雌激素可改善AD大鼠的学习记忆能力。
Objective To evaluate the effect of estrogen on learning and memory ability in rats of Alzheimer＇s disease （AD）. Method 36 female rats were enrolled, and they were randomly divided into the sham operation group （12 cases）, ovariectomy （OVX） group （12 cases） and OVX ＋ estradiol benzoate（EB）group （12 cases）. At first AD rat model was established by injection of amyloidbeta peptidel - 42 （ Aβ1 -42 ） into the hippocampus of rats, Then learning and memory ability was determined by Morris water maze, ELISA was used to test the activity of super oxide dismutase （SOD）, and malondialdehyde（ MDA）, and choline acetyltransferase （CHAT）. Results Compared with OVX group, OVX ＋ EB group had shorter average escape latency, the difference was statistically significant （P 〈 0. 05 ）, and more spenting time in swimming in the target in probe test,the difference was statistically significant （P 〈 0. 05）, and crossing times, the difference was statistically significant （ P 〈 0. 05 ）. The activities SOD and ChAT were heighted, and the MDA content was decreased, the difference was statistically significant （ P 〈 0. 05 ）. Conclusion EB improves cognitive deficits in ovariectomy AD rats.
出处
《吉林医学》
CAS
2016年第9期2122-2124,共3页
Jilin Medical Journal
基金
广州医科大学校基金[项目编号:2013A34]
