摘要
目的：分析某地区献血人群乙型肝炎病毒（HBV）携带者基因型和耐药性基因变异的情况。方法选取本市2014年4月1日-12月31日22539份无偿献血者血液标本，使用ELISA双试剂检测筛查出201份双阳性标本，然后通过荧光定量PCR筛查出99例HBV阳性标本，对其中45例病毒载量大于1000 IU/ml的标本进行HBV耐药基因检测（PCR测序法）。分析本市HBV携带者基因型与耐药片段变异情况及其关联。结果45例全部为乙肝野生型，无耐药突变。乙肝分型结果显示45例中有43例为B型，占95.6%，2例为C型，占4.4%。乙肝B型的分型率明显高于C型的分型率，具有统计学意义（χ^2=9.256，P＜0.05）。结论本市献血人群中HBV携带者的基因分型以B型为主，耐药性基因主要是野生型，荧光定量PCR阳性的标本中有45.5%的病毒载量高于1000 IU/ml，需要跟踪治疗，为预防和治疗乙型肝炎病毒感染提供可靠依据。
Objective To analyze the HBV poisonous genotype and its mutation of drug resistance in blood donation population in a given area. Methods Selected the blood examples from donors since April 1, 2014 to December 31, 2014 and screened out 201 double positive specimens using ELISA double reagent. And then by fluorescence quantitative PCR, 99 cases of HBV positive specimens were screened out. 45 of them held a viral load larger than 1 000 IU/ml specimens, their HBV drug resistance gene were detected （PCR sequencing method）. Finally, we analyzed the relationship between the hepatitis b patient virus poisonous genotype and pills resistance segment mutation of this city. Results All 45 cases was Hepatitis B wild-type, no drug resistance mutation was found. Hepatitis B parting with 45 cases of type B was 43 cases, classification rate was 95.6%, the other 2 cases was type C, classification rate was 4.4%. Type B gene classification rate was significantly higher than C gene classification rate, with statistical significance （χ^2 =9.256,P〈0.05）. Conclusion The city＆#39;s Hepatitis B blood donators hold virus genes model B type at large and the resistance genes are mainly wild＆amp;nbsp;type, 45.5% of them has a virus viral load high to 1 000 IU/ml, which need track treatment, providing prevention and cure references for Hepatitis B.
出处
《临床输血与检验》
CAS
2016年第5期448-451,共4页
Journal of Clinical Transfusion and Laboratory Medicine
关键词
乙型肝炎病毒
基因型
变异
耐药性
Hepatitis b virus
Genotypes
Mutation
Drug resistance
