摘要
高粱是一种抗旱性较强的禾谷类作物。本研究在高粱中克隆到一个全长为693 bp的编码ATP合成酶E亚基的基因(SbATPase-E)。在高粱幼苗期,SbATPase-E基因受Na Cl和脱落酸(ABA)处理诱导上调表达。该基因在拟南芥中过量表达可提高转基因植株的耐旱性和耐盐性,在逆境胁迫条件下转基因拟南芥植株较野生型植株根系发达,可能是转基因植株耐旱性和耐盐性提高的主要原因。在干旱胁迫条件下,转基因植株中DREB2A、P5CS1、RD29A、RAB18和ABI1基因的表达量相对于野生型植株中的表达量提高更为显著;在高盐处理条件下,转基因植株中SOS1和SOS2基因的表达量也较野生型植株中的表达量明显提高。这些抗逆相关基因的上调表达可能是转基因植株抗逆性提高的主要分子机制。
Sorghum（ Sorghum bicolor（ L. a gene encoding ATP synthase subunit E with ） Moench）is a cereal crop with strong drought tolerance. In this study 230 amino acids was cloned in sorghum. The SbATPase-E gene was induced by NaCl and ABA treatment in seedling stage. Heterologous over-expression of SbATPase-E could enhance drought and salt tolerance in Arabidopsis thaliana （L.）Heynh. Further study found that the improved drought and salt tolerance in transgenic plants was associated with more larger root systems in transgenic lines compared with the wild type plants. Moreover, the expression levels of DREB2A, P5CSI, RD29A, RABI8 and ABH in the transgenic plants under drought conditions were higher than that in the wild type, and the transcript levels of SOS1 and SOS2 in the transgenic plants under high salt treatment were also higher. The up-regulation of these stress response genes may suggest the molecular mechanism of stress resistance of SbATPase-E transgenic plants.
出处
《植物遗传资源学报》
CAS
CSCD
北大核心
2016年第5期897-905,共9页
Journal of Plant Genetic Resources
基金
农业部转基因专项(2014ZX08003-004)
国家自然科学基金重点项目(U1138304)
中国农科院创新工程和河北省自然基金项目(C2012407002)
关键词
高粱
拟南芥
ATP合成酶
耐旱性
耐盐性
sorghum
Arabidopsis thaliana （ L. ） Heynh
SbA TPase-E
drought tolerance
salt tolerance
