摘要
目的:建立蒙药材库页悬钩子的指纹图谱,为蒙药材库页悬钩子的质量控制提供依据。方法:采用HPLC技术,Phenomsil ODS(250 mm×4.6 mm,5μm)色谱柱,以甲醇-0.3%磷酸为流动相梯度洗脱,检验波长:290 nm,流速:1.0 ml·min-1,进样量:20μl;测定了7批不同产地、不同采收期库页悬钩子的指纹图谱,得到13个共有峰。结果:建立了各蒙药库页悬钩子药材共有峰的相对保留时间的RSD均小于3%,相对峰面积RSD均小于3%,符合指纹图谱相关要求。结论:建立的HPLC指纹图谱有较好的精密度、重现性和稳定性,可以作为蒙药库页悬钩子质量评价主要依据之一。
Objective： To establish an HPLC fingerprint spectrum of Mongolian medicine Rubus sachalinensis. Methods： An HPLC was performed on the column of phenomsil ODS （250mm×4.6mm,5μm） with the mobile phase of 0.3% phosphoric acid- MeOH with gradient elution at a flow rate of 1.0 ml·min^-1 , the detection wavelength was 290 nm, the column temperature was 25℃and the injection volume was 20μl. Totally 7 batches of Rubus sachalinensis from different habitats were analyzed. Results ： The RSD of relative retention time of the common peaks of Rubus sachalinensis was less than 3% , and that of relative peak areas was below 3% as well, which were in accordance with the requirements of fingerprint. Conclusion： The established HPLC fingerprint has promising accuracy, repeatability and stability, which can be used as one basis for the quality control of Rubus sachalinensis.
出处
《中国药师》
CAS
2016年第9期1662-1665,共4页
China Pharmacist
基金
内蒙古自治区科技计划项目(编号:kjt15sf24)
