摘要
目的：建立隐睾模型，观察分析乙醛脱氢酶1A1（ALDH1A1）mRNA和蛋白在大鼠隐睾及正常睾丸组织中表达的变化及意义。方法应用内分泌诱导法建立隐睾动物模型，分别取出生后第15天（幼年期）、第45天（青春期）和第90天（成年期）隐睾和正常子代雄鼠的睾丸组织，采用HE染色、实时荧光定量聚合酶链反应（ PCR）法、蛋白质印迹法、组织芯片免疫组化检测大鼠隐睾及正常睾丸组织中 ALDH1A1 mRNA和蛋白水平表达的变化。结果隐睾组幼年期、青春期和成年期ALDH1A1 mRNA表达量分别为1.01±0.19，1.60±0.32，0.75±0.16；正常组各期表达量分别为1.66±0.23，0.52±0.08，0.15±0.10，幼年期隐睾组睾丸组织中ALDH1A1 mRNA和蛋白的表达量低于正常组，青春期和成年期隐睾组睾丸组织中ALDH1A1 mRNA和蛋白的表达量高于正常组，差异有统计学意义（P＜0.05）。结论 ALDH1A1在大鼠睾丸的发生发育过程中，在不同年龄阶段有着不同的表达。 ALDH1A1的表达与隐睾疾病的发生发展机制有着重要的联系。
Objective To study the mRNA and protein expression of aldehyde dehydrogenase 1A1 （ALDH1A1）in rat cryptorchidism and normal testis.Methods We established the cryptorchidism model by flutamide and took normal testis as normal group.The testicular tissue samples were collected on 15 days, 45 days, and 90 days after birth respectively.The expression of ALDH1A1 in rat cryptorchidism and normal testis were investigated by real-time PCR, Western blot and immunohistochemisty intissue microarray.Results The mRNA expression of ALDH1A1 in cryptorchidism group in infant , adolescent and adult period were 1.01 ±0.19,1.60 ±0.32,0.75 ±0.16, and 1.66 ±0.23,0.52 ±0.08,0.15 ±0.10 in normal group,respectively.The expression of ALDH1A1 in cryptorchidism group was significantly lower than that in normal group in infant period , but it was significantly higher than that in the normal group in adolescent and adult period （ P〈0.05 ）.Conclusions The expression of ALDH1A1 was different in different age period during the process of testicular development of rat.It showed an important relationship between ALDH1A1 and cryptorchidism.
出处
《中华医学杂志》
CAS
CSCD
北大核心
2016年第34期2744-2748,共5页
National Medical Journal of China
基金
江苏省南通市科技计划项目（HS2014010）,南通大学研究生科技创新计划项目（YKS14002）
