摘要
目的 建立隐睾模型,设正常睾丸组织作为对照,探讨大鼠隐睾组织中同源盒基因-A10（HOXA10）基因mRNA和蛋白表达的特点.方法 手术建立24只单侧隐睾大鼠动物模型,分别于术后第7天（幼鼠期,B7组）、术后第60天（性成熟期,B60组）取出未降睾丸和对侧正常睾丸组织,后者作为对照组（A7组、A60组）.采用实时荧光定量PCR法及免疫组化法检测隐睾模型中HOXA10的mRNA及蛋白表达水平.结果 HOXA10在B7、B60组隐睾侧睾丸组织中表达量低于同期对照组,B60组隐睾侧睾丸组织表达量低于B7组,B7、B60中HOXA10的mRNA的表达量分别减少到0.78±0.11（P＜0.01）和0.56±0.03（P＜0.01）.结论 HOXA10在隐睾中的表达比正常发育的睾丸表达量显著降低;且随着隐睾时间的增加,HOXA10表达降低更明显.
Objective To explore the effects of HOXAIO gene expression in undescended and descended testis. Methods Twenty-four male Sprague Dawley rats were surgically prepared for unilateral cryptorchidism model. Their undescended and descended testes were removed at days 7 （ infancy period, group B7） and 60 （ sexual maturity period, group B60 ） post-operation. And contralateral descended testis served as controls （group A7, group A60 ）. The expression of HOXA10 with histologic changes in experimental cryptorchidism were detected with one-step real-time quantitative reverse transcription- polymerase chain reaction （RT-PCR） and immunohistochemisty. Results Compared with the control group, the levels of HOXA10 gene for groups B7 and B60 decreased markedly to 0.78 ± 0.11 （P 〈 0.01 ）and 0. 56 ± 0. 03 （ P 〈 0.01 ） respectively, However the levels of HOXA10 gene and protein in group B60 were significantly lower than those in group B7 （ P 〈 0.01 ）. Conclusions There is a lower expression of HOXA10 in undescended testes than in normally developed counterparts. And the expression of HOXA10 decreases with the elapsing time of cryptorchidism.
出处
《中华医学杂志》
CAS
CSCD
北大核心
2014年第6期464-466,共3页
National Medical Journal of China
基金
2011年江苏省南通市社会发展项目（HS2011047）
关键词
睾丸
基因表达
同源盒
隐睾
Testes
Gene expression
Homebox
Cryptorchidism
