摘要
目的:探讨鼻咽癌组织中MKK4蛋白的表达与-1044A/T多态性的相关性。方法:90例鼻咽癌患者,运用免疫组化法测定MKK4蛋白的表达,以聚合酶链反应-限制性片段长度多态性法(PCR-RFLP)检测其基因-1044A/T位点单核苷酸多态性。结果:鼻咽癌组织中MKK4蛋白表达(-)为24.4%(22/90),(+)为15.6%(14/90),(++)为34.4%(31/90),(+++)为25.6%(23/90)。低表达(-/+)患者共36例,-1044AA基因型占22例(61.11%),AT基因型占12例(33.33%),TT基因型占2例(5.56%),AT+TT基因型共占14例(38.89%);高表达患者共54例,其中AA基因型占38例(70.37%),AT基因型占15例(27.78%),TT基因型占1例(1.85%),AT+TT基因型共占16例(29.63%)。低表达与高表达发生T基因变异无统计学意义(Z=0.323,P=0.747)。结论:MKK4蛋白表达的高低与-1044A/T启动子基因多态性无明显相关性。
Objective： Discussion MKK4 protein expression in nasopharyngeal carcinoma and-1044A/T polymorphism correlation. Methods： 90 patients with nasopharyngeal carcinoma,MKK4 protein expression was determined by immunohistochemical staining,polymerase chain reaction-restriction fragment length polymorphism（ PCR-RFLP） to detect the gene-1044 A / T sites monocytes nucleotide polymorphism. Results： MKK4 protein expression in nasopharyngeal carcinoma（-） was 24. 4%（ 22 /90）,（ ＋） was 15. 6%（ 14 /90）,（ ＋ ＋） was 34. 4%（ 31 /90）,（ ＋ ＋ ＋） was 25. 6%（ 23 /90）. Low expression（- / ＋） patients with a total of 36 cases,-1044 AA genotype accounted for 22 cases（ 61. 11%）,AT genotype accounted for 12 cases（ 33. 33%）,TT genotype accounted for two cases（ 5. 56%）,AT ＋ TT gene type accounted for 14 cases（ 38. 89%）. The patients with high MKK4 expression of 54 cases,of which accounted for 38 cases of AA genotype（ 70. 37%）,AT genotype accounted with 15 cases（ 27. 78%）,TT genotype accounted for one case（ 1. 85%）,AT ＋ TT genotype accounted for 16 cases（ 29. 63%）. Low expression and high expression of T gene mutation occurs no significant（ Z = 0. 323,P = 0. 747）. Conclusion： MKK4 protein expression correlated with-1044 A / T gene promoter polymorphisms was no significant correlation.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
2016年第8期1137-1140,共4页
Chinese Journal of Immunology
基金
湖北省自然科学基金面上项目(2014CFB675)
湖北省教育厅重点项目(D20141205)
2012年宜昌市科技研究与开发项目(A12301-03)
