摘要
目的观察高脂膳食对青春期雄性大鼠下丘脑、垂体、睾丸结构及性激素水平的影响,并探讨6周自由转轮运动的干预效果。方法 3周龄初断乳雄性SD大鼠40只,随机分为普通膳食对照组(C组)、普通膳食运动组(CE组)、高脂膳食组(D组)、高脂膳食运动组(DE组)。C、D组安静饲养8周,CE、ED组在适应性喂养2周后进行6周自由转轮运动,每天上、下午各1 h,每周5 d。8周后,大鼠腹主动脉取血,测血清T、E2、FSH、LH;制备睾丸组织匀浆液测T、E2;制备下丘脑、垂体、睾丸组织切片,光镜下观察其结构变化。结果 (1)与C组比较,D组血清T、E2检出率降低,且睾丸组织中T水平显著降低(P<0.05),而血清E2、FSH、LH显著升高(P<0.05);下丘脑出现空泡脂滴,垂体中嗜酸性和嗜碱性细胞均减少,睾丸生精小管面积、精子细胞比例和睾丸间质细胞数量降低。(2)与D组相比,DE组大鼠血清T检出值及睾丸T值均增高,而血清E2、FSH、LH显著降低(P<0.05);下丘脑组织结构出现空泡脂滴,垂体中嗜酸性细胞数量显著增高,嗜碱性细胞数量无变化,睾丸组织中生精小管面积较高但差异无显著性。结论 6周自由转轮运动可有效改善高脂饲养大鼠导致的性激素分泌异常,雌雄激素比例失衡,但对下丘脑-垂体-睾丸轴的组织结构病理变化改善效果不明显。
Objective To observe the effects of high fat diet and 6-week voluntary wheel running on the structure of hypothalamus,pituitary and testis and sex hormone levels in pubertal male rats. Methods Forty 3-week-old male Sprague-Dawley rats were randomly divided into following groups： the control group fed with normal diet（ C）,training group fed with normal diet（ CE）,control group fed with high fat diet（ D）,and training group fed with high fat diet（ DE）,10 rats in each group. The groups C and D were bred for 8 weeks freely,and the groups CE and DE were assigned to have voluntary wheel running twice / d,1 h / time,5 d / w,for 6 weeks after a 2-week adaptive feeding. Eight weeks later,blood sample was collected to detect the serum T,E2,FSH and LH,and the E2 and T of testis were also detected. The histology of hypothalamic,pituitary,and testis tissues was observed by light microscopy. Results（ l） Compared with the group C,the group D had significantly decreased levels of serum T and E2 and testicular T（ P 〈 0. 05）,and significantly increasedserum E2,FSH and LH levels（ P 〈 0. 05）. In the group D,vacuolar lipid droplets were increased in the hypothalamus,eosinophils and basophils were reduced in the pituitary,and the area of seminiferous tubules,percentage of sperm cells,and quantity of Leygid cells were significantly decreased in the testis.（ 2） Compared with the group D,the serum T and testicular T concentrations were increased,but the serum E2,FSH and LH were decreased significantly（ P 〈 0. 05） in the group DE,and vacuolar lipid drops were increased in the hypothalamus,eosinophil cells were increased in the pituitary gland,and the area of seminiferous tubules was increased but not significantly in the testis. Conclusions 6-week voluntary wheel exercise can improve the high fat diet-induced abnormal secretion of sex hormones,but not effectively improve the histological changes in hypothalamus,pituitary gland and testis.
出处
《中国实验动物学报》
CAS
CSCD
北大核心
2016年第4期381-387,共7页
Acta Laboratorium Animalis Scientia Sinica
基金
国家自然基金青年项目(编号:30800541)
关键词
高脂膳食
自由转轮运动
性激素
HPT轴
青春期
SD大鼠
High fat diet
Voluntary wheel exercise
Sex-hormones
Hypothalamic-pituitary-testicular axis
Puberty
Sprague-Dawley rats
