摘要
目的观察三黄泻心汤"炮制配伍"解热优势方药效并初步探究其作用机制。方法大鼠皮下注射20%干酵母混悬液复制发热模型,阿司匹林(0.1g·kg^(-1))作为阳性对照药,三黄泻心汤解热优势方分为3个剂量(1、0.5、0.25 g生药·kg^(-1)),灌胃给药。首先测量给药前基础体温,然后分别于给药后0.5、1、2、4 h测量大鼠肛温,以实测体温-基础体温(ΔT)表示体温变化,实验结束后采血及分离血清,取下丘脑组织后制备上清。Elisa法测定血清和下丘脑组织中PGE2、c AMP、Cox-1、Cox-2的含量。结果三黄泻心汤"炮制配伍"解热优势方各剂量组均可使体温下降,并降低发热大鼠血清和下丘脑组织中PGE2、cAMP、Cox-1、Cox-2的含量。结论三黄泻心汤"炮制配伍"解热优势方有明显的解热作用,其机制可能是与抑制PGE_2、c AMP等中枢发热介质的释放有关。
Objective To observe the antipyretic efficacy of Sanhuangxiexin decoction by processing compatibility and explore the mechanisms preliminarily. Methods The fever models were established by subcutaneous injection with dried yeast suspension. Aspirin was used as the positive control （0.1 g · kgl）, and Sarthuangxiexin decoction was divided into three doses （1, 0.5, 0.25 g crude drug · kg-l）, which was intragastrically administrated. Firstly the basal body temperature was measured before administration, then a measurement of the fever temperature was carried out after 0.5, 1, 2, 4 h, which was expressed as AT. The blood was sampled to separate and collecte the serum, and the hypothalamus tissues were taken to prepare supematant from rats after the experiment. Finally, Elisa method was used to test the content of PGE2, cAMP, Cox-l, Cox-2 both in the serum and hypothalamus. Results All three doses of Sanhuangxiexin decoction could decrease the temperature and the content of PGE2, cAMP, Cox-l, Cox-2 in serum and hypothalarnus. Conclusion The formula 8 of Sanhuangxiexin decoction has obvious antipyretic effect, and its mechanisms maybe relate to inhibition of the release of eentric febrile mediators such as PGE： and cAMP, etc.
出处
《中国药物警戒》
2016年第8期449-451,463,共4页
Chinese Journal of Pharmacovigilance
基金
国家自然基金面上项目(81274087)
关键词
炮制配伍
三黄泻心汤
解热
机制
processing compatibility
Sanhuangxiexin decoction
antipyretie
mechanism
