摘要
目的:检测并分析蛋白4.1R基因敲除小鼠(4.1R-/-)和野生型小鼠(4.1R+/+)血液生理生化参数。方法:摘眼球法采集两组小鼠(n=6)血液,用全自动血液细胞分析仪和生化分析仪检测血液生理生化参数。结果:血液生理参数中,4.1R-/-小鼠红细胞计数、血红蛋白、红细胞压积和中性粒细胞比率显著低于野生型小鼠(P<0.05);血小板计数、平均血小板体积、血小板压积、白细胞计数、淋巴细胞比率显著高于野生型小鼠(P<0.05)。生化参数中,4.1R-/-小鼠碱性磷酸酶显著低于野生型小鼠,总胆红素、直接胆红素和间接胆红素显著高于野生型小鼠(P<0.05)。结论:蛋白4.1R缺失可导致慢性溶血性贫血,4.1R-/-小鼠相关血液生理生化参数随之改变。
Aim： To detect and analyze blood physiological and biochemical parameters of 4. 1R gene knockout mice（ 4. 1R- /-） and wild-type（ 4. 1R＋ / ＋） mice. Methods： Blood samples of 4. 1R- /-mice and wild-type mice were collected by enucleating of eyeball,followed by blood physiological and biochemical parameters testing with automatic blood cell analyzer and biochemical analyzer. Results： Compared with those of the wild-type mice,RBC,HGB,HCT,and NEU of4. 1R- /-mice were obviously lower（ P 0. 05）; while PLT,MPV,PCT,WBC and LYM of 4. 1R- /-mice were obviously higher（ P 0. 05）. ALP,one of biochemical parameters,was significantly lower,while T-BIL,D-BIL and I-BIL of 4. 1R- /-mice were significantly higher（ P 0. 05）. Conclusion： Deletion of protein 4. 1R might result in chronic hemolytic anemia,and changes of blood physiological and biochemical parameters may be basic information for the further study with4. 1R- /-mice model.
出处
《郑州大学学报（医学版）》
CAS
北大核心
2016年第4期466-469,共4页
Journal of Zhengzhou University(Medical Sciences)
基金
国家自然科学基金资助项目81172784
30972707
关键词
4.1R
血液生理生化参数
溶血性贫血
小鼠
protein 4
1R
blood physiological and biochemical parameter
hemolytic anemia
mouse
