摘要
目的：本实验通过观察甘草酸二铵注射液对大鼠背部超长随意皮瓣成活的影响,初步探讨其可能机制。方法：健康SD大鼠32只,雌雄不限,体重175~250 g,随机分为实验组和对照组（n=16）,在大鼠背部制作以尾端为蒂的8 cm×2 cm（长宽比4∶1）大小的超长随意皮瓣。术前10 min及术后连续6 d,实验组予甘草酸二铵2 mg/100 g qd腹腔注射,对照组予等量5%葡萄糖注射液腹腔注射。于术后24 h每组随机选择8只大鼠处死,取皮瓣组织检测超氧化物歧化酶（superoxide dismutase,SOD）、丙二醛（malonyldialdehyde,MDA）。术后第7 d处死其余大鼠,计算皮瓣成活率,取皮瓣组织检测SOD、MDA,并取皮瓣近、中、远段进行HE染色,在光镜下观察皮瓣组织学改变。结果：术后第24 h,实验组皮瓣SOD含量高于对照组（P〈0.01）,MDA含量低于对照组（P〈0.01）。术后第7 d,实验组皮瓣成活率高于对照组（P〈0.05）,SOD含量高于对照组（P〈0.01）,MDA含量低于对照组（P〈0.01）。组织学观察显示,实验组近、中、远段皮瓣与对照组相比,组织水肿程度较轻,炎性细胞浸润较少,血管扩张增生较明显,坏死程度较轻。结论：甘草酸二铵能改善大鼠背部超长随意皮瓣的成活率,其作用机制可能与减少氧自由基生成,减轻炎性细胞浸润有关。
Objective： To investigate the effects and the potential mechanism of Diammonium Glycyrrhizinate on the survival of dorsal random skin flap with large length-to-width ratio in rats. Methods： Thirty-two healthy SD rats（male/female） weighing 175 ~ 250 g were randomly divided into the experimental group and the control group（n = 16）. A dorsal random skin flap measured 8 cm × 2 cm（length：width = 4：1）with a caudally based pedicle was performed on each rat. Experimental group were intraperitoneally injected with Diammonium Glycyrrhizinate（2 mg/100 g·qd） ten minutes before the operation and on day 6 after the operation respectively.Control group were intraperitoneally injected with 5% glucose solution with the same dose at the same time points as in the experiment group. The general conditions and changes of the flap were observed every day after operation. At the 24 th hour after operation, 8 rats selected randomly from each group were sacrificed for their flap tissues to be tested for superoxide dismutase（SOD）, and malonyldialdehyde（MDA）. At the 7th day after operation,the rest were sacrificed to calculate flap survival ratio. Meanwhile, flap tissues were obtained not only to test SOD,and MDA levels, but also to do histological observation through light microscope after HE staining.Results： At the24 th hour after operation, compared with the control group, the experimental group showed higher SOD levels（P〈0.01） and lower MDA level（P〈0.01）. At the 7th day after operation, compared with the control group, the experimental group showed higher flap survival ratio（P〈0.05）, higher SOD level（P〈0.01） and lower MDA level（P〈0.01）. Histological observation showed that in the experimental group, compared with the control group, the edema, inflammatory cells infiltration and necrosis were lighter, while the dilation and proliferation of blood vessels were more obvious. Conclusion： Diammonium Glycyrrhizinate can improve the survival of dorsal random flap with large
出处
《泸州医学院学报》
2016年第4期321-325,共5页
Journal of Luzhou Medical College
关键词
甘草酸二铵
皮瓣
缺血再灌注损伤
超氧化物歧化酶
丙二醛
Diammonium glycyrrhizinate
Flap
Ischemia-reperfusion injury
Superoxide dismutase
Malonyldialdehyde
