摘要
烟气中苦味化合物多为难挥发成分,种类多、含量低。本研究采用感官导向鉴定法与液相色谱-高分辨质谱法(LC-HRMS)相结合分析烟气苦味成分,利用凝胶色谱分离主流烟气粒相物水溶性成分,并对各流分进行感官评价以确定苦味流分,合并后得到苦味特征组分。采用Q-Exactive型LC-HRMS仪器分析苦味特征组分,通过保留时间和二级质谱定性,对化合物标准品水溶液进行味觉评价,确定苦味化合物。在苦味特征组分中共鉴定出16种化合物,其中有苦味特征的是新烟草碱、降烟碱、麦斯明、可替宁、2-丁基咪唑、2-异丙基咪唑、烟酰胺、N-甲基烟酰胺、N-乙基烟酰胺和3-乙基-4-甲基-3-二氢吡咯-2-酮。该方法可为鉴定烟气中关键的苦味成分,实现卷烟产品的定向设计,提高卷烟的感官舒适性提供参考。
The bitter compounds in cigarette smoke have non-volatility, diversity and low-abundance. Sensory-oriented separation followed with liquid chromatography cou-pled with high resolution mass spectrometry （LC-HRMS） was proved as a feasible solu-tion for exploring unknown bitter compounds. In this research, water-soluble particu-late of cigarette smoke was separated by gel chromatography into several fractions, which were tasted by six experienced reviewers to orient the bitter fractions. Then the bitter fractions were combined to obtain bitter-characteristic component. Sixty-five com-pounds existing both in this component and in any bitter fraction were preliminarily found with Q-Exactive, a LC-HRMS instrument, using retention time and accurate molecular weight as filter. Sixteen structural formulas of these compounds were con-firmed with LC-MS/MS, using retention time and secondary mass spectra of supposed standard substances as references. Finally the proved standard compounds were dis-solved in water and tasted by six experienced reviewers. The result shows that ten bitter compounds in bitter-characteristic component were confirmed by more than half of them, which are anatabine, nornicotine, myosmine, cotinine, 2-n-butylimidazole,2- isopropylimidazole, nicotinamide, N-methylnicotinamide, N-ethylnicotinamide and3- ethyl-4-methyl-3-pyrrolin-2-One. This method is helpful to identify the key bitter ingredients of cigarette smoke, directionally design cigarette products so as to improve the sensory comfort.
出处
《质谱学报》
EI
CAS
CSCD
北大核心
2016年第5期414-421,共8页
Journal of Chinese Mass Spectrometry Society
基金
国家自然科学基金委青年科学基金项目(21405177)
国家烟草专卖局项目(110201202008)
河南中烟项目(ZW2014058)资助
关键词
苦味化合物
烟气
感官导向鉴定
液相色谱-高分辨质谱(LC-HRMS)
bitter compounds
cigarette smoke
sensory-oriented identification
liquid chromatography coupled with high resolution mass spectrometry （LC-HRMS）
Q-Exactive
