摘要
目的：探讨尿激酶（Urokinase,UK）对大鼠肺成纤维细胞凋亡的影响。方法：5只SD雄性大鼠气管内灌注博莱霉素造肺纤维化模型,将模型组肺成纤维细胞与不同浓度的尿激酶培养24-96h,流式细胞术测定细胞凋亡。结果：尿激酶以时间和浓度依赖的方式抑制肺成纤维细胞生长,流式细胞术检测到凋亡小体。结论：尿激酶能抑制肺成纤维细胞增殖,与凋亡有关。
Objective：To study the effect of urokinase on apoptosis of pulmonary fibroblasts in rats.Methods：Five SD male rats were modeled pulmonary fibrosis modelling by endotracheal perfusion bleomycin.Pulmonary fibroblasts in the model group were cultured with urokinase of different concentrations for 24-96hours.Cell apoptosis was examined by flow cytometry.Results：Urokinase inhibited pulmonary fibroblasts in time and concentration dependent manners.Apoptosis bodies were found by flow cytometry.Conclusion：Urokinase can inhibit the proliferation of fibroblast,which is related to cell apoptosis.
出处
《陕西医学杂志》
CAS
2016年第9期1110-1111,共2页
Shaanxi Medical Journal
基金
陕西省科技研究发展计划(2014KCT-23)
陕西省西安市科技计划项目[SF1316(1)]
