摘要
This paper aims to provide better guidance for construction of trehalose-producing recombinant strains to further improve the yield of trehalose. The research progress on trehalose biosynthesis pathways and the application of molecular biology technique in trehalose study in recent 30 years, especially the last 10 years are reviewed. Results show that there are 5 pathways of trehalose synthesis. Although enzymes and genes of trehalose synthesis have been isolated and genetic engineering strains have increased gradually, the improvement of trehalose yield is still inadequate because most recombinant strains are limited to study the physicochemical properties of single enzyme. With the development of modern biological technology, especially the rapid development of DNA recombinant technology, metagenomics and synthetic biology, high expression of heterologous trehalose in recombinant strains would become a hot research topic in the future.
为了更好地指导海藻糖基因工程菌株的构建,进一步提高海藻糖的产量,综述了国内外近30年,尤其是最近10年海藻糖合成途径及分子生物学方面的研究,表明不同的生物中共存在5条海藻糖合成途径,虽然各途径中的酶与基因已基本分离到,工程菌株的构建也渐次展开,但大部分工程菌局限于用来研究途径中单一酶的理化性质,对海藻糖产量提高方面的研究还存在不足。随着现代生物技术尤其是DNA重组技术、宏基因组学与合成生物学的迅速发展,构建工程菌进行异源高效表达将成为今后海藻糖研究的一个热点。
基金
2015河南省科技创新杰出人才项目(154200510025)
2014河南省基础与前沿技术研究计划项目(14230041321)
2016河南省科学院基本科研业务费项目:几株微生物中抗菌活性物质分离纯化与结构分析~~
