摘要
目的研究双氢青蒿素(dihydroartemisinin,DHA)对人胃癌细胞(SGC-7901、HGC-27)生长抑制和促凋亡的作用。方法用WST-1方法检测DHA处理后的胃癌细胞增殖情况,流式细胞术检测细胞周期和凋亡情况,细胞划痕实验检测细胞的迁移能力。结果不同浓度的DHA可以抑制胃癌细胞(SGC-7901、HGC-27)的增殖,且呈现浓度依赖性增长趋势。DHA作用48 h后,胃癌细胞的周期阻滞在S期,同时有诱导胃癌细胞凋亡的作用;DHA作用24 h和48 h后,对胃癌细胞的迁移能力有抑制作用。结论 DHA可有效地抑制胃癌细胞的增殖,并促进其凋亡。
Objective To investigate the growth inhibitory and apoptosis effects of DHA on gastric cancer cells.Methods The proliferation of gastric cancer cells was detected using WST-1.The cell cycle progression and cell apoptosis were determined by flow cytometry.The cell migration ability was detected by wound-healing assay.Results The different concentrations of DHA inhibited the proliferation of gastric cancer cells（ SGC-7901,HGC-27） in a concentration- dependent manner.DHA arrested the cell cycle of gastric cancer cells at S phase and promoted the apoptosis of gastric cancer cells at 48 h.After treatment with DHA for 24 h and 48 h,the metastasis ability of gastric cancer cells were declined.Conclusion DHA can effectively inhibit the proliferation of gastric cancer cells and promote cell apoptosis.
出处
《广东药学院学报》
CAS
2016年第4期466-469,共4页
Academic Journal of Guangdong College of Pharmacy
基金
国家自然科学基金项目(81171447)
广东省科技计划项目(2014A020212303)
广东大学生科技创新培育专项资金项目(pdjh2016b0262)
