摘要
为了更好地理解水稻早熟性的遗传机制,对隐性早熟突变体ref进行了遗传分析和基因定位。突变体ref对光周期不敏感,与6个晚熟品种(系)杂交F1代均为晚熟。ref×嘉禾218 F2分离群体抽穗期呈现连续双峰分布,早抽穗和晚抽穗之比符合1∶3的分离比,表明ref早熟性主要受1对隐性早熟基因控制。F2群体中1 005株早熟和晚熟单株组成定位群体,混合基因池分析发现5号染色体上SSR标记RM7302和RM3853与突变体ref早熟基因连锁。构建5号染色体的连锁图谱,进行表型和标记基因型的连锁分析,进一步确认突变体ref早熟基因定位在标记RM7302和RM3853之间18.32 c M的遗传区间内。
To uncover the genetic mechanism regulating early flowering in rice,a newly identified mutant ref was genetically analyzed and mapped for earliness genes. R e f was insensitive to photoperiodic response. The F, progenies,derived from ref and six late flowering accessions, expressed late flowering. The heading date in ref×Jiahe218 F2 population displayed continuous and bimodal frequency distributions,and the ratio early flowering plants to late flowering plants followed a 1-3 segregation ratio, suggesting that earliness was controlled by a pair of recessive genes. A total of 1 005 early/late flowering plants derived from F2 generation was selected to construct the gene mapping population. Bulked segregation analysis identified SSR markers RM7302 and RM3853 on chromosome 5 associated with earliness genes. With further linkage analysis,earliness gene was finally mapped to an interval of 18. 32-cM region between SSR markers RM7302 and RM3S53 on the genetic map of chromosome 5.
出处
《江苏农业学报》
CSCD
北大核心
2016年第4期721-724,共4页
Jiangsu Journal of Agricultural Sciences
基金
嘉兴市科技项目(2010AZ2017
2011AZ1021
2012AY1053
2013BZ26001)
浙江省公益项目(2013C32028)
关键词
水稻
隐性早熟突变体
抽穗期
遗传分析
基因定位
rice （ Oryza sativa L .）
recessive earliness
heading date
inheritance
gene mapping
