摘要
目的:探讨藏药二十五味驴血丸对胶原诱导性关节炎大鼠的治疗作用及其机制。方法:选用雌性SD大鼠60只,按随机数字表法分为空白对照组和造模组,再将造模成功的大鼠随机分为CIA模型组、甲氨蝶呤组、二十五味驴血丸低、中、高剂量组,连续给药4周。结果:与模型组比较,二十五味驴血丸使CIA大鼠的AI评分和肿胀肢体的脚掌厚度显著降低,并可使CIA大鼠血清中IL-17A的含量显著降低,使TGF-β1含量显著升高。结论:二十五味驴血丸对胶原诱导的大鼠关节炎具有显著的治疗效果,其可能通过调控Th17/Treg细胞平衡从而达到治疗类风湿关节炎的作用。
Objective： To explore the therapeutic effect and mechanism of tibetan compound prescription of Ershiwuwei lvxue pills on Collagen-Induced Arthritis（ CIA） rats. Method： Eight rats were randomly selected as blank control group from sixty SD female rats which were six weeks old,and other rats as Rheumatoid arthritis model group. The successful model rats were randomly devided into CIA group,positive drug（ MTX） group,low dose ershiwuwei lvxue pills group,medium dose ershiwuwei lvxue pills group and high dose ershiwuwei lvxue pills group. Every group was eight rats. The blank control group and the CIA model group were fed with equal volume of double distilled water for four weeks. The contents of IL-17 A and TGF-β1 in serum were measured by ELISA after the rats were killed. Result： Compared with CIA group,the AI scores and paw thickness of the Ershiwuwei lvxue pills groups obviously decreased. Compared with CIA group,the level of IL-17 A in serum of ershiwuwei lvxue pills groups decreased obviously while the level of TGF-β1increased evidently. Conclusion： Ershiwuwei lvxue pills has a obviously therapeutic effect on CIA rats and may play the role of the treatment of RA through regulating the balance of Th17 / Treg cells.
出处
《中国中医基础医学杂志》
CAS
CSCD
北大核心
2016年第8期1053-1056,共4页
JOURNAL OF BASIC CHINESE MEDICINE
