摘要
为探讨表没食子儿茶素没食子酸酯（EGCG）对白介素-4（IL-4）刺激的巨噬细胞精氨酸酶1（Arg-1）和诱导型一氧化氮合成酶（iNOS）表达的影响.将6~8周龄Balb/c小鼠经无血清DMEM培养基刺激后,收集腹腔巨噬细胞用于体外培养.体外培养的巨噬细胞经20ng/mL IL-4和不同浓度EGCG处理后,用实时荧光定量PCR（RTqPCR）和酶联免疫吸附试验（ELISA）方法检测Arg-1和iNOS的mRNA表达水平和含量.结果显示IL-4和EGCG单独处理均可显著刺激Arg-1的表达和抑制iNOS的表达,而EGCG（12.5~50μM）增强IL-4刺激的Arg-1表达和IL-4对iNOS表达的抑制作用,且存在剂量依赖效应.上述结果提示EGCG以剂量依赖方式促进IL-4刺激的Arg-1表达、抑制iNOS的表达.
To investigate the influence of epigallocatechin gallate （EGCG）, a main active constituent in gree tea polyphe- nals, on the expression of arginase-1 （Arg-1） and inducible nitric oxide synthase （iNOS） stimulated by interleukin-4 （IL-4） in vitro murine peritoneal macrophages. The Balb/c mice with 6-8 weeks age were stimulated with serum-free DMEM medium through intraperitoneal injection,and peritoneal macrophages were collected to culture. After the cultured macrophages in vitro were treated with 20 ng/mL IL-4 and various dosage EGCG, respectively, both mRNA level and protein content of Arg-1 and iNOS were detected with RT-qPCR and ELISA in cultured macrophages in vitro. The results show that 20 ng/mL IL-4 or/and 12.5 ~ 50 vmol/L EGCG alone facilitated the Arg-1 expression and suppressed iNOS expression, but EGCG up-regulated the Arg-1 expression elicited by IL-4 and down-regulated the iNOS expression, in dose-depended fashion. These results suggested that EGCG regulated the arginine metabolism in routine macrophages in vitr.
出处
《河南师范大学学报（自然科学版）》
CAS
北大核心
2016年第4期101-105,共5页
Journal of Henan Normal University(Natural Science Edition)
基金
河南省科技攻关计划项目(122102310282)
