摘要
建立了一种合浦珠母贝血细胞原代培养方法,为贝类免疫学研究提供实验平台。通过台盼兰染色法检测细胞活力,结果显示:7d内细胞生长状态稳定,并且培养基中的FBS对细胞生长活力没有显著影响。以细胞中iNOS活性为检测指标,详细研究了LPS和IL-2对合浦珠母贝血细胞的激活作用。结果表明:LPS和IL-2对iNOS活性的诱导作用表现出浓度和时间依赖的特征。刺激后36h左右iNOS活性达到最大,LPS和IL-2的最佳诱导剂量范围分别为1～10g/mL和10～100ng/mL,并且IL-2对iNOS活性的激活作用较LPS快。
A useful experimental system for primary hemocytes cultures using pearl oyster （Pinctaclafucata） is established, Seven days after initiation of the culture, the viability of hemocytes remained constant as measured by the trypan blue exclusion technique. Survival of cells cultured in FBS-supplemented L-15 medium was similar to that of cultured in L-15 medium not containing FBS. The effects of LPS and IL-2 on the pearl oyster hymocytes cultures were examined through determination of the iNOS activities. LPS and IL-2 were found to induce the iNOS activities in a dose and time-dependent manner. The best response in the activity of iNOS was obtained after about 36 hours of stimulation, and the optimized concentrations of LPS and IL-2 were 1-10 μg/mL and 10-100 ng/mL, respectively. The results also indicate that iNOS activity would be induced more rapidly by IL-2 than by LPS.
出处
《科技导报》
CAS
CSCD
2007年第13期45-48,共4页
Science & Technology Review
基金
国家高技术研究发展计划(863计划)项目(2006AA09Z413
2006AA09Z441)
