摘要
为提升生物有机肥料中功能微生物的数量,腐熟堆肥中添加外源氨基酸固态发酵功能菌是研制生物有机肥的主要手段。目前主要外源添加氨基酸菜粕的高成本严重阻碍了生物有机肥产业的发展。本试验通过研究酸解羽毛粉作为外源添加蛋白对固态发酵功能菌的影响及其所研制生物有机肥的促生效果,以开发生物有机肥新的原料配方。研究结果表明,腐熟堆肥中,随着酸解羽毛粉添加量的增加,所研制生物有机肥中功能菌的数量呈现先增加后下降趋势,为促进功能菌的增殖,酸解羽毛粉最优添加量为50 g/kg;盆栽试验结果表明,酸解羽毛粉作为外源蛋白添加制成的生物有机肥能够显著增加茄子和番茄株高、茎粗和SPAD值,移栽20天时,相比于对照,茄子分别增加19.02%、29.02%和4.80%;移栽50天时,番茄分别增加14.74%、18.70%和4.74%。研究结果表明,酸解羽毛粉作为外源蛋白添加剂能够有效增殖固体发酵过程中的功能菌,研制的低成本生物有机肥具有优异的促生效果,研究结果能够为生物有机肥产业的发展提供理论依据。
In order to improve the quality of bio-organic fertilizer (BIO), addition of amino acids to assist with the reproduction of the functional microbe inoculated into matured composts though solid-state fermentation is demonstrated to be an efficient way. An addition of significant quantity of oil rapeseed cakes could lead to a much better growth of microbial species added during the BIO preparation, however, the higher production costs hindered severely the large-scale extension of BIOs. This study investigated the possibility to produce bio-organic fertilizer (BIO) by using hydrolyzed feather powders as a solid-state fermentation (SSF) medium. The results showed that the biomass of functional strain increased with the increasing amount of hydrolyzed feather powders added in the matured compost with best additive amount of 50 g/kg. Pot experiments showed that the produced BIO efficiently promoted the growths of eggplant and tomato; compared with the control, the new produced BIO increased plant height, stem diameter and SPAD values by 19.02%, 29.02% and 4.80%, respectively, after transplanting for 20 days for eggplant; and by 14.74%, 18.70% and 4.74%, respectively, after transplanting for 50 days for tomato. In conclusion, supplemented with hydrolyzed feather powders has certain advantages in solid-state fermentation of the PGPR strain and can produce high quality hio-organic fertilizer for promoting plant growth. The above results provide the theoretical and technical supports for the development of BIOs.
出处
《土壤》
CAS
CSCD
北大核心
2016年第4期661-667,共7页
Soils
基金
国家高技术发展计划(863计划)项目(2013AA102802)
中央高校基本科研业务费专项资金项目(KYZ2015197和KYCYL201502)
国家自然科学基金项目(31572212)
南京市科技计划项目(201505041)
江苏省固体有机废弃物资源化高技术研究重点实验室开放资金项目(BM201101301)
青蓝工程项目资助
关键词
酸解羽毛粉
生物有机肥
促生
Hydrolyzed feather powder
Bio-organic fertilizer
Plant growth promotion