摘要
目的综合分析结肠癌组织中转录因子CCAAT/增强子结合蛋白-α(C/EBPα)的表达,并研究C/EBPα的表达对结肠癌SW480细胞系侵袭性的影响。方法选取2014年1月至2015年9月在该院确诊为结肠癌的36例患者临床资料,利用免疫组织化学法分析36例结肠癌患者的结肠癌组织和正常组织中的C/EBPα表达情况。将结肠癌组中的C/EBPα表达情况设为研究组,正常组织中的C/EBPα表达情况设为对照组,每组均为18例。采用SPSS17.0统计学软件进行统计学分析,分析C/EBPα的表达与结肠癌临床病理参数之间的关系。在此基础上,利用蛋白免疫印迹法(Western blot)发现SW480细胞系为高表达C/EBPα肠癌细胞株;构建pCDGFP-C/EBPα真核表达质粒,通过细胞划痕实验研究其迁移能力的变化,并检测与肿瘤侵袭相关蛋白(KLF5、MMP-2、MMP-9、ECD)表达量的相关性。结果免疫组织化学法的相关研究结果显示,在正常组织中,C/EBPα的低表达率为6.21%;在结肠癌组织中,C/EBPα的低表达率为67.84%,两组数据比较差异有统计学意义(P<0.05)。Western blot结果显示,低表达C/EBPα的患者的肿瘤直径越大、TMN分期越晚,过表达C/EBPα的SW480细胞的KLF5、MMP-2、MMP-9表达越低,而E钙黏蛋白(ECD)表达越高。结论低表达C/EBPα与结肠癌的TMN分期和转移有着密切关系,过表达C/EBPα能够显著降低结肠SW480癌细胞的侵袭性,对未来的研究方向有着重要的临床研究价值。
Objective To analyze the expression of C/EBPα in colon tissue and to study the impact of expression C/EBPα on in-vasion of SW480 cell lines .Methods Clinical data of 36 patients with colon cancer from January 2014 to September 2015 were col-lected ,and C/EBPα expression was detected by immunohistochemistry .C/EBPα expression condition in colon tissue was treated as test group ,and the condition in the normal tissue was treated as control group ,each group had 18 cases .Using SPSS17 .0 statistical software ,relationship between the expression and pathological parameters was analyzed .Meanwhile ,by Western blot ,SW480 C/EBPα cancer cell lines were found to be high expression cell lines .Eukaryotic expression plasmid of pCDGFP-C/EBPα was built ,and by cell scratch test （cell wound scratch assay） ,changes of migration and its relationship with tumor invasion protein （KLF5 ,MM P-2 ,MMP-9 and ECD） were detected .Results The results of immunohistochemistry showed that in normal tissues ,low expression rate of C/EBPα was 6 .21% ,and low expression rate in colon tissue was 67 .84% ,difference was statistically significant （ P〈0 .05） .Western blot analysis showed that the greater of diameter of tumor in patients with low expression of C/EBPα ,the later sta-ges of TMN as well as the higher expression of KLF5 ,MMP-2 and MMP-9 in over-expression of C/EBPα of SW480 cells ,while the lower expression of ECD .Conclusion The low expression of C/EBPα is closely related to TMN staging and metastasis of colon cancer .Overexpression of C/EBPα can significantly reduce the SW480 invasion of colon cancer cell ,which has important clinical val-ue in the future research .
出处
《检验医学与临床》
CAS
2016年第16期2294-2296,共3页
Laboratory Medicine and Clinic
