摘要
采用两种不同方法对玉米变性淀粉基因组DNA提取比较,并经特异性引物进行实时荧光定量-聚合酶联反应(qRT-PCR)检测,从中选择出更适合玉米变性淀粉后续转基因成分检测的DNA提取方法。通过采用磁珠法和试剂盒法提取玉米变性淀粉基因组DNA,比较不同方法的提取效果。结果表明,试剂盒法的提取效果优于磁珠法。实时荧光定量PCR结果显示,试剂盒法提取的基因组DNA均可扩增出标准的S形曲线,而磁珠法提取的基因组DNA则无任何扩增信号。该研究为检测玉米变性淀粉中的转基因成分奠定了基础。
The different methods of genome DNA from modified maize starch were compared. The specific primers were detected by quantitative re- al-time polymerase chain reaction （qRT-PCR）, and the DNA extraction method which was more suitable for subsequent gmo detection modified maize starch was determined. The genome DNA from modified maize starch was extracted by paramagnetic particle and kit method, and the extrac- tion effects of different methods were compared. The results showed that the genome DNA extracted by kit method could be amply standard S-shaped curve, but the genome DNA extracted by magnetic beads method could not find any amplification signal. This study established the founda- tion for detection of genetically modified components from modified maize starch.
出处
《中国酿造》
CAS
北大核心
2016年第8期53-56,共4页
China Brewing
基金
中国南方智谷引进创新团队项目(201209)
关键词
玉米变性淀粉
DNA提取
转基因成分
检测
modified maize starch
DNA extraction
genetically modified components
detection
