摘要
本试验在间接ELISA的基础上建立了一种检测抑制素(inhibit hormone,INH)表位多肽疫苗抗体的方法并对ELISA试剂盒进行优化,为今后测定主动免疫后细毛羊体内INH表位多肽疫苗抗体提供理论参考。在前人研究的基础上,采用间接ELISA法测定血清中INH表位多肽疫苗抗体的含量,通过对不同试验条件的控制摸索出最佳试验条件,即以脱脂奶粉为封闭液,INH及GnIH以表位多肽疫苗抗体稀释度为20 000倍,最佳反应时间60min,最佳显色时间为15min。本试验建立了一种检测细毛羊体内INH及GnIH抗体的方法,为今后对INH表位多肽疫苗抗体的研究提供了参考。
In this study,an indirect ELISA method was established to detect inhibin hormone（INH）epitope peptide vaccine antibody,it would provide oretical reference for the determination of Fine-wool sheep after active immune body INH epitope peptide vaccine antibody.On the basis of the predecessors,using indirect ELISA method to determinate serum INH epitope peptide antibody levels of sheep,and through control different experimental conditions to look for the best experimental conditions.Through explorating the experimental conditions,finally,the testing experiment conditions were determined,which was blocked solution with skimmed milk powder,INH and GnIH synthetic peptides dilution degrees for 20 000 times,the optimum reaction time was 60 min,the best color action time was 15 min.In this experiment,a kind of method to detection antibody in the body after INH active immune sheep was built,it would provide a reference for future research.
出处
《中国畜牧兽医》
CAS
北大核心
2016年第8期2156-2163,共8页
China Animal Husbandry & Veterinary Medicine
基金
甘肃省青年自然基金“非繁殖季节GnIH基因免疫对藏羊卵泡发育的调节机制”
国家绒毛用羊产业技术体系分子育种岗位(CARS-40-03)
中国农业科学院“细毛羊资源与育种”创新工程(CAAS-ASTIP-2015-LIHPS)
关键词
抑制素
细毛羊
主动免疫
生殖激素
inhibin hormone
Fine-wool sheep
active immunization
reproductive hormone
