摘要
目的探究CD4+T细胞及淋巴亚群在小儿哮喘与肺炎中的水平及临床意义。方法选取东营市广饶县中医院儿科自2013年9月至2015年9月收治的34例哮喘患儿作为哮喘组,33例肺炎患儿作为肺炎组,另选择同时期的正常体检小儿30例作为对照组,3组均在早晨空腹情况下抽取静脉血5m L,并于3小时内进行标本处理,后采用流式细胞技术检测外周血中CD4+T细胞内细胞因子T细胞辅助细胞(Th)1、Th2、Th17及外周血中CD4+CD25+FOXP3+调节性T细胞的水平。结果与对照组相比,哮喘组CD4+CD25+FOXP3+、CD4+IL-4+Th1及CD4+IL-17+Th17细胞百分比均增加明显,差异均具有统计学意义(t值分别为5.34、5.89、6.11,均P<0.05)。与对照组相比,肺炎组CD4+CD25+FOXP3+细胞百分比降低,差异具有统计学意义(t=4.56,P<0.05)。与肺炎组相比,哮喘组CD4+CD25+FOXP3+、CD4+IL-4+Th1、CD4+IL-17+Th17细胞百分比均增加,差异均具有统计学意义(t值分别为6.12、6.45、6.39,均P<0.05)。与对照组相比,哮喘组淋巴细胞水平降低,单核细胞、嗜酸性粒细胞水平升高,差异均具有统计学意义(t值分别为5.34、5.26、5.19,均P<0.05)。与对照组相比,肺炎组单核细胞水平升高,差异具有统计学意义(t=4.99,P<0.05)。与肺炎组相比,哮喘组嗜酸性粒细胞水平升高,差异具有统计学意义(t=5.29,P<0.05)。结论 CD4+T细胞及淋巴细胞亚群检测在诊断与鉴别小儿哮喘与肺炎疾病时具有重要临床意义,为临床治疗提供参与依据。
Objective To probe into and analyze CD4＋T cells and lymphocyte subsets levels in children with asthma and pneumonia and their clinical significance. Methods From September 2013 to September 2015 34 children with asthma were selected in asthma group and33 children with pneumonia in pneumonia group from Dongying City Guangrao County Hospital. At the same period another 30 normal children taking physical examination were chosen in control group. Before breakfast 5m L blood were sampled from venous for all cases in three groups in the morning and processed within 3 hours. Then flow cytometry was used to detect Th1,Th2 and Th17 in CD4＋T cells and levels of CD4＋CD25＋FOXP3＋in peripheral blood. Results Compared to the control group,cell percentages of CD4＋CD25＋FOXP3＋,CD4＋IL-4＋Th1 and CD4＋IL-17＋Th17 increased in the asthma group with significant differences（ t value was 5. 34,5. 89 and 6. 11,respectively,all P〈0. 05）. Compared to the control group,CD4＋CD25＋FOXP3＋cell percentage decreased in the pneumonia group with significant difference（ t = 4. 56,P〈0. 05）. Compared to the pneumonia group,in the asthma group CD4＋CD25＋FOXP3＋,CD4＋IL-4＋Th1,CD4＋IL-17＋Th17 cell percentages increased with significant differences（ t value was 6. 12,6. 45 and 6. 39,respectively,all P〈0. 05）. Compared to the control group,lymphocyte levels dropped,monocytes and eosinophils levels increased in the asthma group with significant differences（ t value was 5. 34,5. 26 and 5. 19,respectively,all P〈0. 05）,and monocytes levels increased in the pneumonia group with significant difference（ t = 4. 99,P〈0. 05）. Compared to the pneumonia group,eosinophil levels increased in the asthma group with significant difference（ t = 5. 29,P〈0. 05）. Conclusion CD4＋T cells and lymphocyte subsets detection is of great importance in the diagnosis and identification of pediatric asthma and pneumonia disease,which provides basis for clinical treatment.
出处
《中国妇幼健康研究》
2016年第7期861-863,共3页
Chinese Journal of Woman and Child Health Research
