摘要
以新疆阿勒泰采集的灵芝子实体XJ-001为试材,采用组织分离纯化获得该菌纯培养,以菌丝体基因组DNA为模板进行ITS片段扩增,测序并进行系统发育分析;以5种不同培养基培养菌株,通过比较生长速度,选出适宜母种培养基;摇瓶发酵研究不同碳氮源对菌株菌丝体生物量、胞内及胞外多糖含量的影响。结果表明:根据形态特征结合rDNA-ITS分析,初步将XJ-001鉴定为Ganoderma lucidum,并获得GenBank登录号为KT997431;菌株在玉米粉、豆饼粉琼脂培养基长势最优,生长速度为18.7mm·d^(-1);以葡萄糖、酵母浸粉为最适碳、氮源,在转速150r·min^(-1),30℃培养5d,菌株XJ-001菌丝体生物量、胞内/外多糖含量分别为8.15、1.18、0.05mg·mL^(-1)。该菌株具有生长迅速和产糖较高的潜在性能,可为开发新疆野生灵芝资源提供参考。
Fruiting body of a wild Ganoderma lucidum XJ-001 was obtained from Altay of Xinjiang as the material,with the method of organization separation,the mycelium was isolated and purified from the fruiting body.Genomic DNA of strain XJ-001 was extracted from mycelium,and then used as template for the PCR amplification of ITS fragment.ITS fragment was purified and sequenced.The sequence was analyzed with phylogenetic analysis method.XJ-001 was cultured on five different medias.Optimal mother culture medium was selected by calculating mycelium growth rate.The effect of different carbon and nitrogen sources on mycelium biomass,intracellular and extracellular polysaccharide contents of the strain were studied by shake flask fermentation.The results showed that according to the morphological characteristics combined with rDNA-ITS analysis,the strain XJ-001 was identified as Ganoderma lucidum and GenBank accession number of the ITS was KT997431.The corn soybean agar medium was chosen as excellent mother culture medium,growth rate was 18.7mm·d^（-1）.The strain XJ-001 was cultured at 30℃and 150r·min^（-1） for 5days using glucose and yeast extract as the carbon and nitrogen source,vespectively.Mycelium biomass,intracellular and extracellular polysaccharide contents were 8.15mg·mL^（-1）,1.18mg·mL^（-1） and 0.05mg·mL^（-1）,respectively.The results showed that the strain XJ-001 had the potential property to grow rapidly and produce high yield of polysaccharide.The results of this research would provide a reference for the development of Xinjiang wild Ganoderma lucidumresources.
出处
《北方园艺》
CAS
北大核心
2016年第15期142-146,共5页
Northern Horticulture
基金
新疆农业科学院青年科技基金资助项目(xjnkq-2014013)
关键词
新疆灵芝
鉴定
碳氮源
Xinjiang Ganoderma lucidum
identification
carbon and nitrogen sources
