摘要
目的采用多聚酶链反应-变性梯度凝胶电泳(polymerase chain reaction-denaturing gradientgel electrophoresis,PCR-DGGE)技术研究中药复方人参乌梅汤加味对腹泻大鼠肠道菌群构成特征的影响。方法 48只SD大鼠随机抽取12只为空白组,其余36只参照文献方法以复合因素制作腹泻模型。造模成功后随机分为模型组、西药组、中药组,每组12只。造模21d开始给药干预,空白组和模型组予生理盐水,其余各组分别给予妈咪爱、人参乌梅汤加味,均1次/d,连续给药7d。收集各组大鼠粪便排泄物,提取粪便细菌总基因组DNA,PCR扩增细菌16Sr DNA基因V3可变区,DGGE方法检测PCR产物。对优势条带进行回收测序,鉴定菌种。结果药物处理后腹泻模型大鼠肠道微生物相对丰度最高的为厚壁菌门类细菌,其次为拟杆菌门和变形菌门类细菌。人参乌梅汤加味治疗后大鼠肠道厚壁菌门类细菌含量显著增加(P<0.01),而肠道拟杆菌门类细菌含量显著降低(P<0.01),肠道变形菌门类细菌含量无显著变化(P>0.05)。与空白组相比,经人参乌梅汤加味治疗后大鼠菌群较之西药组更接近空白组。结论人参乌梅汤加味较之西药对肠道菌群平衡更具有良性的调控作用,可能通过影响肠道菌群结构,纠正菌群紊乱,进而发挥生津止泻效应。
Objective It is to study the influence of compound ginseng wumei decoction on the Characteristics of the diarrhea model rats intestinal flora by polymerase chain reaction- denaturing gradient gel electrophoresis technology. Methods Randomly selected 12 from 48 SD rats for the control group,and the remaining 36 according to the literature method were made into diarrhea model by combined factors. After the success of the building were randomly divided into model group,western medicine,Chinese medicine group,each group of 12. Building 21 d to drug intervention,the control group and model group to saline,the rest group were given mammy love,ginseng wumei decoction,the average of 1 / d,continuous administration 7d. Collect all rats feces in each group,extract fecal bacteria genomic DNA,PCR amplified bacteria 16 S r DNA gene V3 variable region. DGGE method for detecting PCR products. Advantages bands were recovered for sequencing and identification of bacteria species. Results drug treatment of diarrhea model rats after the highest relative abundance of intestinal microbial was Firmicutes bacteria categories,followed by Bacteroidetes and Proteobacteria categories. Ginseng wumei decoction in rats after treatment intestinal Firmicutes bacteria contentincreased significantly（ P〈0. 01）,while the intestinal bacteroidetes bacteria content decreased significantly（ P〈0. 01）,Proteobacteria categories showed no significant change（ P〉0. 05）. Compared with the western medicine group,after ginseng wumei decoction rat microbiota much closer to the control group. Conclusion Compared with western medicine Ginseng wumei decoction can take beneficial action on the intestinal flora balance. Ginseng wumei decoction may influence the structure of the intestinal flora to correct flora disturbance,and then develop fluid antidiarrheal effect,
出处
《时珍国医国药》
CAS
CSCD
北大核心
2016年第7期1548-1551,共4页
Lishizhen Medicine and Materia Medica Research
基金
国家自然科学基金(No.81102544)
