摘要
目的评价多次七氟醚麻醉对新生大鼠的神经毒性作用。方法SPF级健康sD大鼠32只，雌雄不拘，7日龄，体重15～20g。采用随机数字表法分为2组（n=16）：对照组（c组）和多次七氟醚麻醉组（Sev组）。Sev组于出生后7、14和21d时吸入2．6％七氟醚2h，C组吸入空氧混合气体。于出生后32～36d行Morris水迷宫实验测定认知功能，分别于出生后21和36d时每组随机取8只大鼠麻醉后取脑脊液，采用ELISA法测定B淀粉样蛋白的浓度。结果与c组比较，Sev组逃避潜伏期、平台区有效区域运动时间、运动距离、有效区域进入次数、滞留时间百分比、运动距离百分比及进入次数百分比差异元统计学意义（P〉0．05），出生后36d时脑脊液B淀粉样蛋白浓度升高（P〈0．05）。结论多次七氟醚麻醉对新生大鼠可产生轻度中枢神经毒性作用，但未引起认知功能变化。
Objective To evaluate the neurotoxieity induced by muhiple exposures to sevoflurane anesthesia in the neonatal rats. Methods Thirty-two healthy SPF Sprague-Dawley rats of both sexes, aged 7 days, weighing 15-20 g, were randomly divided into 2 groups （n=16 each） using a random number table： control group （group C） and multiple exposures to sevoflurane anesthesia group （group Sev）. On postnatal day 7, 14 and 21, 2.6% sevoflurane was inhaled for 2 h in group Sev, while the mixed gas of oxygen and air was inhaled instead of sevoflurane in group C. Morris water maze test was carried out on postnatal day 32-36 to assess the cognitive function. On postnatal day 21 and 36, 8 rats in each group were selected and anesthetized, and the cerebrospinal fluid was collected for determination of the concentrations of amyloid β-protein by enzyme-linked immunosorbent assay. Results Compared with group C, no significant change was found in the escape latency, movement time spent in the effective region of the platform, movement distance, the number of entries into the effective region, percentage of residence time, percentage of movement distance, and percentage of the number of entries （P〉0.05） , and the concentrations of amyloid S-protein in the cerebrospinal fluid were significantly increased on postnatal day 36 in group Sev （P〈 0.05）. Conclusion Multiple exposures to sevoflurane anesthesia can induce central neurotoxicity, but do not induce changes in the cognitive function in the neonatal rats.
出处
《中华麻醉学杂志》
CAS
CSCD
北大核心
2016年第4期411-413,共3页
Chinese Journal of Anesthesiology
基金
国家自然科学基金（81360180）
贵州省科技厅联合基金（黔科合J字LKZ[2012]04号）
关键词
麻醉药
吸入
婴儿
新生
药物毒性
Anesthetics,inhalation
Infant,newborn
Drug toxicity
