摘要
目的:观察石斛合剂含药血清对H_2O_2诱导SH-SY5Y细胞氧化应激的影响及可能的机制。方法:体外培养的SH-SY5Y细胞预先予以石斛合剂含药血清(10%)孵育24 h,加入终浓度为400μmol·L-1的H_2O_2培养1 h,采用MTT法测细胞存活率、酶法测细胞内SOD活力、MDA和GSH含量、流式细胞术测细胞凋亡情况等,以观察石斛合剂含药血清对H_2O_2诱导SH-SY5Y细胞氧化应激损伤的影响。结果:与模型组比较,石斛合剂含药血清能明显提高受H_2O_2刺激的SH-SY5Y细胞的存活率(P<0.05),降低MDA水平和提高SOD和GSH活力(P<0.01),并减少因H_2O_2引起的神经细胞凋亡。结论:石斛合剂可保护H_2O_2引起的SH-SY5Y细胞氧化应激损伤并抑制其凋亡。
Objective： To observe the effect of Dendrobium Compound（ DC） on oxidative stress in SH-SY5 Y cells induced by H2O2 and study its possible mechanism. Methods： Hatch SH-SY5 Y cells in vitro with DC-containing serum（ 10%） in advance for 24 h,and add H2O2（ 400 μmol/L） to culture for 1 h,and then use MTT assay to measure the cell survival rate and enzyme method to measure intra-cellular SOD activity,MDA and GSH content and flow cytometry to test cell apoptosis situation etc.,in order to observe the effect of DC-containing serum on SH-SY5 Y cells oxidative stress injury. Results： Compared to the model group,DC-containing serum could significantly improve the survival rate of SH-SY5 Y cells stimulated by H2O2（ P 〈 0. 05）,decrease the level of MDA and increase the activity of SOD and GSH（ P 〈 0. 01） and reduce the nerve cell apoptosis induced by H2O2. Conclusion： DC can protect SH-SY5 Y cells from oxidative stress damage induced by H2O2 and inhibit cells’ apoptosis.
出处
《中华中医药学刊》
CAS
北大核心
2016年第8期1911-1913,共3页
Chinese Archives of Traditional Chinese Medicine
基金
国家自然科学基金青年科学基金项目(81403442)
福建省自然科学基金项目(2014J01354)
