摘要
目的对中药补骨脂Psoralea corylifolia中的化学成分进行研究,以期发现新的活性化合物。方法运用硅胶、凝胶、MCI-GEL树脂及RP-HPLC等多种色谱技术对补骨脂的化学成分进行分离纯化,并根据理化性质和波谱数据鉴定化合物的结构。结果从补骨脂95%乙醇提取物分离鉴定了1个二氢异黄酮类化合物3R-6-羟基-8-羟甲基-4′,7-二甲氧基-二氢异黄酮()。结论化合物为新化合物,命名为补骨脂异黄酮A(corylisoflavone A);表现出一定的细胞毒活性,其中对急性早幼粒细胞白血病细胞(NB4)、人神经母细胞瘤细胞(SH-SY5Y)、人前列腺癌细胞(PC3)、人肺腺癌细胞(A549)和人乳腺癌细胞(MCF7)的IC50值分别为12.6、5.3、15.5、4.8和11.2μmol/L。
Objective For the purpose of finding new bioactive agents from Chinese herbal medicine,the chemical study on Psoralea corylifolia was carried out.Methods The chemical constituents in the fruits of P.corylifolia were isolated by silica gel,MCI-GEL resin,Sephadex LH-20 column chromatography,and high performance liquid chromatography（HPLC） methods.The structures of compounds were elucidated by spectroscopic methods,including extensive 1D and 2D NMR techniques.Results A new isoflavanone,3R-6-hydroxy-8-（hydroxymethyl）-4＇,7-dimethoxy-dihydrogenisoflavone（1） was isolated from the fruit of P.corylifolia.Conclusion Compound 1 is a new compound named corylisoflavone A,which displays the cytotoxicity against NB4,SH-SY5 Y,PC3,A549,and MCF7 cells with IC50 values of 12.6,5.3,15.5,4.8,and 11.2 μmol/L,respectively.
出处
《中草药》
CAS
CSCD
北大核心
2015年第15期2206-2208,共3页
Chinese Traditional and Herbal Drugs
基金
国家自然科学基金资助项目(21362044,21032085)
云南省高校科技创新团队资助项目(IRTSTYN 2014-11)
关键词
补骨脂
豆科
二氢异黄酮
细胞毒活性
补骨脂异黄酮A
Psoralea corylifolia L.
Leguminosae
isoflavanone
cytotoxicity
corylisoflavone A
