摘要
目的探讨 UC 初发活动期、缓解期和复发后活动期的结肠上皮细胞增殖与凋亡的变化，研究5-氨基水杨酸（5-ASA）在诱导缓解和维持缓解治疗中对结肠上皮细胞增殖和凋亡的影响。方法纳入12例2002年1月至2012年12月进行治疗并长期随访的，应用5-ASA 诱导缓解并长期维持治疗的轻中度 UC 患者，作为 UC 组；选择同期10例行健康体检并接受结肠镜检查未见异常者或仅见单发息肉者作为对照组。调取 UC 组患者初发活动期、缓解期和复发后活动期结肠镜活组织检查病理组织学标本。采用 Ki-67免疫组织化学染色和末端脱氧核苷酸转移酶介导的 dUTP 缺口末端标记法（TUNEL），检测结肠上皮细胞的增殖与凋亡水平。数据以中位数（下四分位数，上四分位数）表示。采用 Kruskal-Wallis 检验、Mann-Whitney 检验比较组间差异，采用 Bonferroni 法修正。结果初发活动期、缓解期和复发后活动期 UC 患者结肠黏膜上皮细胞中位增殖指数分别为31．65％（19．14％，39．66％）、12．30％（11．11％，14．10％）、44．15％（33．65％，52．45％），均高于对照组的7．89％（6．54％，8．86％），多组间差异有统计学意义（H ＝30．033，P ＜0．01），初发活动期和复发后活动期均高于缓解期，差异均有统计学意义（P 均＜0．05／6）。初发活动期、缓解期和复发后活动期 UC 患者结肠上皮细胞的中位凋亡指数分别为24．18％（17．81％，27．16％）、44．19％（43．41％，50．55％）、41．24％（33．78％，46．24％），均高于对照组的2．65％（2．48％，2．98％），组间差异有统计学意义（H ＝31．563，P ＜0．01），其中缓解期和复发后活动期高于初发活动期（P 均＜0．05／6）。对照组、初发活动期、缓解期、复发后活动期中细胞凋亡增殖比分别为0．3200（0．2755，0．4250）、0．7499（0．6349，1．1158）、
Objective To investigate proliferation and apoptosis of colonic epithelial cells from ulcerative colitis （UC）patients with active new-onset,remission and active recurrent stages,and to study the effects of 5-aminosalicylic acid （5-ASA）on proliferation and apoptosis of colonic epithelial cells during the treatment of inducing remission and maintanence remission. Methods From January 2002 to December 2012,twelve patients with mild-to-moderate UC who received treatment and long-term follow-up and achieved remission with 5-ASA and received long-term maintanence treatment with it were assigned to UC group.At the same period,10 healthy individuals with negative endoscopy results or solitary colonic polyp were assigned to control group.The biopsy tissues from colonoscopy for pathological examination of patients in UC group at new-onset active stage,remission stage and recurrence stage were＆amp;nbsp;obtained.Levels of proliferation marker Ki-67 and apoptosis of colonic epithelial cells were determined by immunohistochemistry and terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling （TUNEL）assay,respectively.Data were expressed by median（lower quartile,upper quartile）.Kruskal-Wallis test and Mann-Whitney test were performed to compare the differences between groups and Bonferroni method was used for correction.Results The median proliferation indexes （PI）of colonic epithelial cells of UC patients at new-onset active period,remission period and recurrent active period were 31 .65 % （19.14%,39.66%）, 12.30% （11 .11 %,14.10%） and 44.15 % （33.65 %,52.45 %）, respectively,which were all higher than those of control group （7.89% （6.54%,8.86%））there were statistically significant differentes among four groups （H =30.033,P 〈0.01 ）;those of new onset active period and recurrent active period were both higher than remission period,and the differences were statistically significant （all P 〈0.05/6）.The median apoptosis indexes （AI）of colonic epithelial cel
出处
《中华消化杂志》
CAS
CSCD
北大核心
2016年第7期475-480,共6页
Chinese Journal of Digestion
基金
益普生腹泻研究基金（IDF2007-11）
关键词
结肠炎
溃疡性
5-氨基水杨酸
增殖
凋亡
Colitis, ulcerative
5-aminosalicylic acid
Proliferation
Apoptosis
