摘要
目的:研究肉苁蓉中尿囊素提取物对小鼠的急性毒性作用。方法:采用最大给药量试验进行肉苁蓉中尿囊素提取物急性毒性研究,用浓度6.5 g生药/m L提取液灌胃,灌胃体积20 m L/kg,24 h内给药两次,小鼠最大给药量折合生药为260 g/kg。给药后观察动物所出现的毒性反应,连续观察14 d。观察结束后,对小鼠进行剖检,裸眼下观察小鼠的心、肝、脾、肺、肾等重要器官有无异常,并计算小鼠各脏器系数。结果:在此剂量下小鼠无一死亡,且无任何异常表现。剖检的器官无明显异常发现。给药组脏器系数与体质量与对照组相比,无明显差异(P>0.05)。结论:小鼠最大给药量相当于人临床日用量的1300倍,说明肉苁蓉中尿囊素提取物较为安全,为其临床研究奠定基础。
Objective： To investigate the acute toxicity of allantoin extract from Roucongrong（Herba Cistanches） in mice. Methods： The method of the maximum dosage was used to study the acute toxicity of allantoin extract from Roucongrong（Herba Cistanches）. The mice were fed in the maximum concentration of 6.5 g crude drug/m L and the largest volume of 20 m L/kg twice a day. The maximum dosage was 260 g crude drug/kg.Mice were sacrificed for autopsy after 14 days. The coefficient of heart, liver, spleen, lungs and kidney were observed. Results： There was no significant difference on the acute toxicity test, behavioral characteristics,weight, organs coefficient between the experimental group and the control group. All visceral organs of mice had no significant change（P〉0.05）. Conclusion：The maximum dosage in mice is equal to 1300 times of daily dosage of people. The results show that allantoin extract from Herba Cistanches is safe. And it＇s lay the foundation of clinical study.
出处
《中医药导报》
2016年第16期47-49,共3页
Guiding Journal of Traditional Chinese Medicine and Pharmacy
基金
新疆维吾尔自治区高技术研究发展计划(201417105)
关键词
肉苁蓉
尿囊素提取物
急性毒性
最大给药量
Roucongrong（Herba Cistanches）
allantoin extract
acute toxicity
maximum dosage
