摘要
目的 建立陈皮健胃片中橙皮苷含量的测定方法 。方法 采用高效液相色谱法（HPLC）,色谱条件为Inertsil ODS-SP（4.6 mm×150 mm,5μm）色谱柱分离,检测波长为283 nm,流动相为乙腈-水进行梯度洗脱,流速为1.0 ml/min,柱温为30℃,理论板数≥3000。结果 橙皮苷进样量线性范围为0.10-2.00μg;在精密度试验、稳定性试验、重复性试验中,橙皮苷峰面积的相对标准偏差（RSD）为2.06%、2.12%、2.14%;橙皮苷的加样回收率为97.36%-102.39%,平均值为99.29%,RSD为2.06%（n=9）。结论 本法的重现性和专属性良好,加标回收率符合要求,能准确、稳定地对陈皮健胃片中的橙皮苷含量进行定量测定,可作为该制剂的质量控制方法之一。
Objective To establish a method for the determination of hesperidin in Chenpi Jianwei tablet.Methods A high performance liquid chromatography （HPLC） method was established.The chromatographic conditions were as follows：the chromatographic column Inertsil ODS-SP （4.6mm×150 mm,5μm） with the mixture of acetonitrile and water as mobile phase in gradient mode,the detection wavelength at 283 nm,the flow velocity of 1.0 ml/min,the column temperature at 30 ℃,the theoretical plate number no less than 3000.Results The linear relationship between content and peak area was obtained during the range of 0.10 - 2.00 μg for hesperidin.RSD of the peak area of hesperidin for the precision test,the stability test and the repeatability test was 2.06 %,2.12 % and2.14 % respectively.The recovery rate of hesperidin obtained were in the range of 97.36% 102.39% and the average of 99.29% with RSD of 2.06% （n=9）. Conclusion This method can be applied to quantitative assay of hesperidin in Chenpi Jianwei tablet with good specificity and reproducibility.The sampling recovery test is in compliance with the requirement,which can be applied to the quality control of Chenpi Jianwei tablet.
出处
《中国当代医药》
2016年第20期4-6,14,共4页
China Modern Medicine
基金
广东省江门市科技计划项目(2014020)
关键词
陈皮健胃片
橙皮苷
高效液相色谱法
Chenpi Jianwei tablet
Hesperidin
High performance liquid chromatography
