摘要
目的观察醒脑静注射液对急性脑梗死（ACI）患者外周血单个核细胞（PBMC）中γ-谷氨酰半胱氨酸合成酶（γ-GCS）、谷胱甘肽过氧化物酶（GSH-Px）、过氧化氢酶（CAT）及美国国立卫生研究院卒中量表（NIHSS）评分的影响，探讨其神经功能的保护作用机制。方法选择2015年1月至11月在湖北省荆门市第一人民医院神经内科住院的70例确诊为ACI患者，按随机数字表法分为对照组和观察组，每组35例。两组均进行一般常规治疗，观察组在常规治疗基础上静脉滴注（静滴）醒脑静注射液30mL（加入0．9％生理盐水注射液250mL中），每日1次，连用14d。同时选取本院35例健康志愿者作为健康对照组。治疗前和治疗后7d、14d监测对照组和观察组患者NIHSS评分的变化；比较3组受试者外周血单个核细胞（PBMC）中γ-GCS、GSH-Px和CAT水平；采用Pearson相关分析法分析抗氧化应激因子水平与NIHSS评分的相关性。结果两组治疗后NIHSS评分均较治疗前降低，γ-GCS、GSH-Px、CAT均较治疗前明显升高，且以观察组治疗后14d的变化较对照组更显著[NIHSS评分（分）：6．18±0．36比11．27±1．26，γ-GCS（ng／L）：91．72±30．29比67．51±23．96，GSH-Px（mg／L）：202．25±55．80比185．25±49．93，CAT（kU／L）：230．25±81．29比200．38±72．78：均P〈0．05]；治疗前及治疗后14dNIHSS评分与γ-GCS、GSH-Px和CAT呈显著负相关（治疗前r值分别为-0．696、-0．549、-0．427，P值分别为0．000、0．001、0．015；治疗后r值分别为-0．435、-0．452、-0．406，P值分别为0．011、0．009、0．037）。结论醒脑静注射液可明显改善ACI患者的神经功能缺损，可能与上调γ-GCS、GSH-Px和CAT水平密切相关。
Objective To observe the effects of Xingnaojing injection on γ-glutamylcysteine synthetase （ γ -GCS）, glutathione peroxidase （GSH-Px）, catalase （CAT） in peripheral blood mononuciear ceils （PBMC） and National Institute of Health stroke scale （NIHSS） score of patients with acute cerebral infarction （ACI）, and to investigate the mechanism of protective effect on nerve function. Methods Seventy patients admitted to Department of Neurology in Jingmen No.1 People＇s Hospital from January to November 2015 were enrolled, and they were randomly divided into a control group and an observation group, each 35 cases. Both groups received conventional treatment, and the observation group additionally was given Xingnaojing injection 30 mL ＋ 0.9% normal saline 250 mL intravenous drip, once a day for consecutive 14 days, one therapeutic course. Meanwhile 35 healthy volunteers in our hospital were recruited and assigned as a healthy control group. Before treatment and 7 days and 14 days after treatment, the changes of NIHSS scores were observed in control group and observation group; the levels of γ-GCS, GSH-Px and CAT in PBMC were compared among the three groups. Pearson correlative analytic method was applied to analyze the correlations between the levels of anti-oxygenation stress factors and NIHSS scores. Results The NIHSS score after treatment was lower than that before treatment in the two groups, γ-GCS, GSH-Px, and CAT were significantly higher than those before treatment, and the degrees of changes of above indexes on 14 days after treatment in observation group were more obvious than those in the control group [NIHSS score： 6.18 ± 0.36 vs. 11.27± 1.26, γ-GCS （ng/L）： 91.72 ±30.29 vs. 67.51 ± 23.96, GSH-Px （mg/L）： 202.25 ± 55.80 vs. 185.25 ± 49.93, CAT （kU/L）： 230.25 ± 81.29 vs. 200.38 ± 72.78, all P 〈 0.05]; the correlations between NIHSS score before and 14 days after treatment were negatively correlated with serum levels of γ-GCS, GSH-Px and CAT （the r valu
出处
《中国中西医结合急救杂志》
CAS
北大核心
2016年第4期352-355,共4页
Chinese Journal of Integrated Traditional and Western Medicine in Intensive and Critical Care
基金
湖北省自然科学基金（2015CFC828）
