摘要
通过检测广粉1号根系中与抗性途径有关的信号物质NO、H_2O_2和SA含量变化,揭示枯草芽胞杆菌TR21菌株诱导的粉蕉系统抗性与SAR途径的关系。试验设置清水(CK)、TR21叶腋接种、香蕉枯萎病菌FOC004菌株根系接种和挑战接种(TR21叶腋接种24h后再根系接种FOC004)4种方式处理广粉1号种苗,测定4种方式接种后0、12、24、48、72、96h根系SA、NO、H_2O_2含量。结果显示:TR21处理和FOC004处理均在早期避免激活植物产生NO,而挑战处理则迅速激活植物根系产生NO并持续维持高水平;TR21处理和FOC004处理也在早期避免迅速激活植物产生H_2O_2,FOC004处理在24h才显著激发植物根系H_2O_2含量升高,而TR21处理需要到48h才使根系H_2O_2达到最高,但挑战处理在12h就显著激发了植物根系的H_2O_2,并一直维持较高水平;TR21处理早期显著激发植物根系游离SA的产生并避免植物产生结合态SA,FOC004处理则抑制植物根系在应答早期产生游离态和结合态SA,挑战处理主要激发植物长期稳定高水平产生结合态SA。试验表明,TR21叶腋接种的确可以激发广粉1号后期快速应答病原菌的入侵,对TR21进一步开发和大田生产应用具有意义。
In order to reveal the relationship between systemic acquired resistance（ SAR） and systemic resistance in dwarf banana Guangfen No.1（ Musa sp. ABB） that induced by Bacillus subtilis strain TR21,the signal substances, NO,H_2O_2 and SA in the roots of Guangfen No.1 were detected. The seedlings were treated by four treatments as control （water inoculated in leaf axils）,TR21 inoculated in leaf axils,Fusarium oxysporum f. sp. cubense strain FOC004 microconidia inoculated on roots and challenge inoculation（ TR21 vaccinated leaf axils,24 h later FOC004 inoculated on roots）,and then the SA,NO,and H_2O_2 content in roots were detected at 6 time points after 0 h,12 h,24 h,48 h,72 h and 96 h. Results showed that,both of the TR21 and FOC004 treatments avoided activating the plant to produce NO in early stage,but challenge inoculation activated the plant roots producing NO quickly and maintaining a high level for long time. TR21 and FOC004 avoided activating the plant generating H_2O_2 quickly in early stage,FOC004 only stimulated the H_2O_2 increasing markedly in roots at 24 h,but H_2O_2 in TR21 treated kept low level until 48 h and reached the highest level,the challenge inoculation treatment significantly stimulated the H_2O_2 increasing at the 12 h,and remained at a high level. TR21 treatment only promoted the production of free SA,but avoided bound SA observably in the early stage,FOC004 treatment inhibited the both free and bound SA production,but challenge inoculation motivated the plant producing bound SA for a long-term and stable level. To sum up,TR21 inoculation in leaf axils early could indeed help the Guangfen No.1 response the invasion of pathogen quickly,which would help the further development and field application of TR21.
出处
《广东农业科学》
CAS
2016年第5期101-106,共6页
Guangdong Agricultural Sciences
基金
仲恺农业工程学院研究生创新项目(KA151495007)
2012年省级农业科技推广项目(粤财农[2013]185号)
关键词
香蕉枯萎病
枯草芽胞杆菌
诱导抗性
信号物质
Fusarium oxysporum f. sp. cubense
Bacillus subtilis
induced resistance
signal substances
