摘要
目的探讨高压氧联合艾塞那肽对高糖培养下视网膜神经节细胞（RGCs）的保护机制。方法传代培养RGC-5细胞系,分为对照组、高糖组、高压氧组、艾塞那肽组、高压氧联合艾塞那肽组。用CCK-8法检测高压氧、艾塞那肽对高糖培养下RGC-5细胞活性的影响,用流式细胞仪评估高压氧、艾塞那肽对高糖培养下RGC-5细胞凋亡的影响,通过电镜观察RGC-5细胞线粒体形态的变化。结果在1.4~2.2 ATA压力范围内,不同压力的高压氧预处理均可提高RGC-5细胞存活率,1.6 ATA压力是高压氧保护RGC-5细胞的最佳剂量。高压氧联合艾塞那肽可提高高糖培养下RGC-5细胞存活率,抑制RGC-5细胞凋亡。结论高压氧、艾塞那肽均能保护由高糖引起的RGC-5细胞的损害,而高压氧联合艾塞那肽的保护效果要比单纯高压氧治疗效果好。
Objective To explore the protective effects of hyperbaric oxygen and exenatide on retinal ganglion cells（ RGC） incubated under high glucose and its role in RGC-5 cell protection mechanism. Methods Cultured RGC-5 were divided into 5 groups： the control group,the high glucose group,the hyperbaric oxygen（ HBO） group,the exenatide group and the hyperbaric oxygen combined with exenatide group. The survival rate and apopsis of RGC-5 cell incubated under high glucose influenced by hyperbaric oxygen and exenatide were measured by CCK-8 kit and flow cytometry,respectively. The changes of mitochondrial membrane potential were monitored by confocal laser scanning microscopy. Results From 1. 4 ATA to 2. 2 ATA,different pressures of hyperbaric oxygen treatment could protect RGC-5 cells from injury induced by high glucose. And 1. 6 ATA was the most optimal dose of hyperbaric oxygen. Hyperbaric oxygen pretreatment and exenatide could elevate mitochondrial membrane potential and control cell apopsis. Conclusion Hyperbaric oxygen combined with exenatide therapy can protect RGC from high glucose induced damage and may benefit the patients with diabetic retinopathy. The effect in combined treatment group is better than that in HBO group.
出处
《临床军医杂志》
CAS
2016年第7期681-684,688,共5页
Clinical Journal of Medical Officers
