摘要
目的观察含蓝莓花色苷的谷物发酵物(简称蓝禾,LH)对小鼠的抗疲劳作用。方法雄性昆明小鼠80只,分2批进行实验。(1)第1批实验:昆明小鼠随机分为对照组和低、中、高剂量LH干预3组,每组10只小鼠,其中对照组小鼠灌胃蒸馏水17.5 ml/kg体质量,LH干预组小鼠灌胃剂量分别为8.75、17.5、35.08 ml/kg体质量。连续灌胃28 d,测定小鼠负重游泳时间。(2)第2批实验:昆明小鼠随机分为对照组及高剂量LH干预组,每组20只小鼠,分别灌胃蒸馏水和LH,灌胃剂量均为35.08 ml/kg体质量。连续灌胃28 d,测定小鼠血乳酸(LA)、血清尿素氮(BUN)和肝糖原的含量。结果与结论 (1)与对照组比较,LH干预组小鼠负重游泳时间均显著延长(P<0.01),高剂量LH干预效果最好;(2)LH干预组小鼠游泳后20 min,LA含量呈下降趋势(P>0.05)、BUN含量明显降低(P<0.05)、肝糖原储备量显著增高(P<0.05)。LH具有显著的抗疲劳作用。
Objective To investigate the anti-fatigue activity of fermented grain-containing blueberry anthocyanins（ LANHE,LH） in mice. Methods Experiments were conducted in two phases. In the first phase,forty mice were randomly divided into four groups： control group（ distilled water,dw） and three LH administration groups（ 8. 75,17. 5 and 35. 08 ml / kg body mass）. In the second phase,mice were randomly divided into two groups： high-dose LH group（ 35. 08 ml / kg body mass） and control group（ dw 35. 08 ml / kg body mass）. After four weeks,a forced swimming test was performed and the biochemical parameters related to fatigue were examined. Results and Conclusion The administration groups showed a significant increase of swimming time to exhaustion compared with the control group,especially the high-dose group（ P〈 0. 001）. There was no significant change in the blood lactate between the two groups,but at 20 min after swimming,the lactic acid（ LA） contents of the high-dose group were lower than in the control group（ P〉 0. 05）. LH could significantly increase the liver glycogen contents and decrease the serum contents（ P〈 0. 05）. These data indicate that LH has antifatigue activity and can elevate the exercise tolerance in mice.
出处
《军事医学》
CAS
CSCD
北大核心
2016年第7期558-560,共3页
Military Medical Sciences
基金
军队"十二五"后勤科研计划(BWS12J038)
关键词
含蓝莓花色苷的谷物发酵物
抗疲劳
小鼠
fermented grain-containing blueberry anthocyanins anti-fatigue mice
