摘要
以西南地区不同来源的15个大豆种质资源为研究对象,研究大豆主要化学成分与其田间霉变抗性间的相关性。采用高效液相色谱法测定大豆中12种异黄酮含量,气质联用法测定大豆中19种脂肪酸含量,分光光度法测定大豆皂苷、可溶性多糖、蛋白含量,并进行聚类及相关性分析。结果表明,15个大豆种质资源依据化学成分含量被分为5个类别,C103等材料的品质性状优良,具有较好的田间霉变抗性,可作为优质大豆种质资源进一步开发利用。棕榈酸、反亚油酸和芥酸等脂肪酸组分含量高的大豆材料其霉变程度更高,A环C-6位甲氧基化的GL型异黄酮(黄豆黄素、黄豆黄苷、乙酰黄豆黄苷、丙二酰黄豆黄苷)及异黄酮苷元含量高的大豆具有更大的抗霉变潜力。
Fifteen soybean germplasm resources were tested in this current research, they were collected from different ar- eas of Southwest China. Correlation analyses between the main chemical constituents of the soybean and their field mil- dew resistance were conducted. The quantification of 12 isollavones in soybean seeds were performed using an Agilent 1100-series high performance liquid chromatography（HPLC） system. The content of 19 fatty acids were measured using gas chromatograph-mass spectrometry （GC-MS）. The soyasaponin, soluble polysaecharides and protein content were measured by UV-Vis spectrophotometry. From the result of our research, these fifteen soybean gcrrnplasms were divided into five groups. Soybean seed of CI03 with high quality and better field mildew resistance, it can be used as high-quality soybean germplasm for further exploitation. Soybean seeds with higher mildew degree were rich in palrnitic acid, transli- nolcic acid, erucic acid, etc. GL type of isoflavones （ glycitcin, glycitin, acctylglycitin, malonylglycitin） and aglycone were rich in the soybean gcrmplasms with greater potential resistance effect for field mildew. Those isoflavoncs were methoxy- lation in the C-6 site of A-ring.
出处
《天然产物研究与开发》
CAS
CSCD
北大核心
2016年第7期1001-1007,共7页
Natural Product Research and Development
基金
国家自然科学基金青年科学基金(31401329)
中国博士后科学基金面上项目(2014M560724)
关键词
大豆
脂肪酸
异黄酮
皂苷
田间霉变
soybean
fatty acid
isoflavone
saponin
field mildew
