摘要
对乳酸乳球菌乳脂亚种BTQY-112增殖培养基进行优化研究。经试验确定乳酸乳球菌乳脂亚种BTQY-112最适培养基为:葡萄糖为5 g/L,乳糖为5 g/L,酪蛋白胨3.3 g/L,大豆蛋白胨3.3 g/L,鱼蛋白胨3.3 g/L,β-甘油磷酸二钠28.5 g/L,Tween-80 0.5 g/L,七水硫酸镁0.25 g/L,乙酸钠1.55 g/L,K2HPO40.83 g/L,柠檬酸钠0.62 g/L,抗坏血酸钠1.5 g/L。在此增殖培养基中经30℃,14 h培养活菌数提高了4.3倍。
The optimization of proliferation culture medium for Lactococcus lactis subsp. BTQY-112 was stud-ied.The best medium was got with the composition of 5 g/L glucose, lactose 5 g/L, casein peptone 3.3 g/L, soy-bean peptone 3.3 g/L, fish peptone 3.3 g/L,β-glycerol phosphate disodium salt 28.5 g/L, Tween-80 0.5 g/L, MgSO4·7H2O 0.25 g/L, sodium acetate 1.55 g/L, K2HPO4 0.83 g/L, sodium citrate 0.62 g/L, sodium ascorbate 1.5g/L. After cultivated in enrichment medium for 14 h at 30℃, number of live bacteria increased 4.3 times.
出处
《食品研究与开发》
CAS
北大核心
2016年第12期159-162,共4页
Food Research and Development
