摘要
为了获得提取率高的灰树花菌丝体蛋白,以灰树花菌丝体为原料,采用超声波提取灰树花菌丝体蛋白。在单因素试验基础上,采用正交试验设计,以蛋白提取率为评价指标,对提取工艺条件进行优化。结果表明,最佳提取条件为液料比95∶1(m L∶g),超声功率600 W,超声温度35℃,超声时间3 min,p H 10.5,在该条件下,灰树花菌丝体蛋白提取率为(5.10±0.04)%。
In order to obtain high extraction rate of protein from Grifola frondosa mycelium, the G. frondosa mycelium protein was extracted by ultra- sonic wave with the G. frondosa mycelium as raw material. On the basis of single factor experiments, with the extraction rate of protein as the evalua- tion index, the extraction processing was optimized by orthogonal experiments. The results showed that the optimum extraction conditions were liquid-material ratio 95：1 （ml：g）, ultrasonic power 600 W, ultrasonic temperature 35 ℃, ultrasonic time 3 min and pH 10.5. Under the conditions, the protein extraction rate was （5.10±0.04）%.
出处
《中国酿造》
CAS
北大核心
2016年第7期151-154,共4页
China Brewing
基金
国家自然科学基金资助项目(31460537)
关键词
灰树花
菌丝体蛋白
超声波
提取:优化
Grifola frondosa
mycelium protein
ultrasonic wave
extraction
optimization
